Research group  leaders at the BBZ:  Dr. Ronny Schulz  

Research group
leaders at the BBZ:
Dr. Ronny Schulz

Bioreactors

Since 2005, the group is focusing on the differentiation of adult MSCs of different species for the treatment of cartilage and bone defects. It was shown in vitro, that MSCs can differentiate chondrogenically within a clinically used collagen I matrix and that these constructs are suitable for the treatment of osteochondral defect of the knee (Schulz, Zscharnack et al. 2008). In addition, optimizations of the oxygen conditions during in vitro cultivation improved chondrogenic differentiation of MSCs (Zscharnack, Pösel et al. 2009).
Moreover, we investigated the effect of cytokines (IL-1b, IL-4), growth factors (rhTGF-SS3) and matrix-fragments (FN-f, Col-fs) on signal transduction in chondrocytes (Chowdhury, Schulz et al. 2010).

The “Development of a bioreactor system for an in vitro cultivation and stimulation of human chondrocytes” (Schulz et al. 2008 ) was an integral part of the European 5th FP-Project “IMBIOTOR – a machine to process tissue engineered implants” (Shelton et al. 2003).
The developed stand-alone bioreactor system allows for integrated compressive loading and perfusion control of tissue engineered cartilage and bone constructs. The bedside bioreactor complies with guidelines for GMP regulations and has been validated for studies in animal trials involving the regeneration of cartilage in a bioreactor for reimplantation into sheep with traumatic focal cartilage defects and for the therapy of osteochondral defects utilizing pre-differentiated stem cells for osteochondral transplantation. The bioreactor device holds two patents (DE10349484A1  and WO2005040332A3: “Method and bioreactor for the cultivation and stimulation of three-dimensional, vital and mechanically resistant cell transplants”) for 3D culture of transplants.


last update: 16.10.2017 

Contact

Dr. Ronny M. Schulz
Dipl. Ing. (FH), cand. MSc
Universität Leipzig
Centre for Biotechnology and Biomedicine
Deutscher Platz 5
04103 Leipzig

Phone: +49 341 97-31352
Fax: +49 341 97-31359
E-Mail

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