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Thole
Züchner
Position
phone:
eMail:
Address: |
Ph.D.
Group Leader
+49-0341 / 97 313 - 90
Thole Züchner, Ph.D.
Head of Ultrasensitive Protein Detection Unit
Center for Biotechnology and Biomedicine
-Institute for Bioanalytical Chemistry
University of Leipzig
Deutscher Platz 5
04103 Leipzig
Germany |
| 06/07-today: |
Head of
Ultrasensitive Protein Detection Unit
Center for Biotechnology and Biomedicine
Institute for Bioanalytical Chemistry
University of Leipzig |
| 08/05-06/07: |
Post
doctoral fellow
within the Nordic Center of Excellence at the Wallenberg Neuroscience
Center, Lund University,
Sweden / in collaboration with the CMBN, Oslo University, Norway |
| 06/02-06/05: |
PhD
thesis
at the Paul
Flechsig Institute, Leipzig, Germany, Prof. Reinhard Schliebs
and
at the
University of Texas, UTMB, USA, Prof. Regino Perez-Polo,
(05/03 - 11/03 und
05/04 - 06/04)
“Regulation of alpha- and beta-secretases in a model for
Morbus Alzheimer” |
| 10/01-03/02: |
Diploma
thesis: Paul Flechsig
Institute,
Leipzig, Germany
Prof. Reinhard Schliebs, “Receptor-mediated mechanisms
regulating
the expression of alpha- and beta-secretases in Morbus
Alzheimer” |
| 10/99-09/01: |
Postgraduate
studies of
biochemistry, University
of Leipzig |
| 10/97-09/99: |
Studies
of
biochemistry,
University of Potsdam |
Publications:
Doblaff
K, Kreisig T, Berthold N, Hoffmann R, Zuchner
T.
Novel
peptide-protein assay for identification of antimicrobial peptides by
fluorescence quenching. (2012) Anal Bioanal Chem, PubMed
Simeonov P,
Zahn M, Sträter N, Zuchner T.
Crystal structure of a supercharged variant of the human
enteropeptidase light chain. (2012) Proteins, PubMed
Zauner
T, Berger-Hoffmann R, Müller K, Hoffmann R, Zuchner T.
A novel highly
adaptable and sensitive protease assay based on fluorescence resonance
energy transfer. (2011) Anal
Chem 83, 7356-7363 PubMed
Hagan A, Zuchner
T.
Lanthanide-based
time-resolved luminescence immunoassays
(Review) (2011) Anal Bioanal
Chem 400, 2847-2864 PubMed
Kreisig T, Hoffmann R, Zuchner T.
A
fluorescence-based homogeneous immunoassay detects antigens within 90
seconds. (2011) Anal Chem 83,
4281-4287 PubMed
Simeonov
P, Berger-Hoffmann R, Hoffmann R, Sträter N, Zuchner T.
Surface
supercharged human enteropeptidase light chain shows improved
solubility and refolding yield. (2010) PEDS 24, 261-268 PubMed
Singer D, Zauner
T, Genz M, Hoffmann
R,
Zuchner T.
Synthesis of
pathological and nonpathological human exon 1 huntingtin. (2010) J Pept
Sci. 27;16:358-363 PubMed
Zuchner T,
Schumer F, Berger-Hoffmann
R, Mueller K, Lukas M, Zeckert K,
Marx J, Hennig H, Hoffmann R.
Highly Sensitive Protein Detection Based
on Lanthanide Chelates with Antenna Ligands Providing a Linear Range of
Five Orders of Magnitude. (2009) Anal Chem 81, 9449–9453 PubMed
Staflin K, Zuchner
T.,
Lundberg C.
Identification of proteins involved in neural progenitor
cell targeting of gliomas. (2009), BMC Cancer, 9(1):206 PubMed
Zuchner T.,
Brundin P.
Mutant
huntingtin can paradoxically
protect neurons from death (2008), Cell Death Diff, 15(3):435-42 PubMed
Staflin K., Lindvall M., Zuchner
T., Lundberg C.
Instructive
cross-talk
between neural progenitor cells and gliomas.. (2007) J Neurosci Res,
85(10):2147-59 PubMed
Zuchner
T.,
Kazemi AR., Weigelt J., Starikow J., Tanaka S., Rothstein
S., Brundin P. Determination of the tertiary structure of exon 1
Huntingtin., (Abstract) (2007) J Neurochem 101 (suppl 1): 65 PubMed
Zuchner
T.,
Schliebe N., Schliebs R.
Zinc-uptake is mediated by M1
muscarinic acetylcholine receptors in differentiated SK-SH-SY5Y cells,
(2006) Int J Dev Neurosci 24:23-27 PubMed
Zuchner
T.,
Schliebs R. Perez-Polo J.R.
Downregulation of muscarinic
acetylcholine receptor M2 adversely affects the expression of
Alzheimer´s disease relevant genes and proteins. (2005) J
Neurochem 95:20-32 PubMed
Zuchner
T.,
Perez-Polo J.R., Schliebs R.
Beta-secretase BACE1 is
differentially controlled through muscarinic acetylcholine receptor
signaling. (2004) J Neurosci Res, 77; 2:250-57 PubMed
Zuchner
T.,
Schliebs R.
Expression of beta-secretase BACE is
differentially controlled through muscarinic acetylcholine receptor
signaling. (Abstract) (2003) J Neurochem 87 (suppl 1): 95 PubMed
Articles
in non peer reviewed journals:
T. Kreisig, S. Mairif, T.
Zuchner.
Moderne
Proteindiagnostik: das Potential
zeitaufgelöster Fluoreszenz. (2011) Laborwelt IV/2011: 29-31 pdf
(in German)
R.Muehlbacher, T. Zuchner.
Protein detection in gels and on membranes. (2008) Screening -
Trends in drug discovery Vol. 9: 4:30-31 pdf
Selected
(invited)
Talks:
University
of Bergen, Sars Seminar Series, Bergen, Norway, 2010
XIV.
International Symposium on Luminescence Spectrometry, Prague,
Czech Republic,
2010
International
Congress on Analytical Proteomics, Lisbon, Portugal, 2009
University
of
Texas Medical Branch, Galveston, USA 2009
Athens University Ohio, USA, 2009
European
Conference on Metallobiolomics, Berlin, Germany, 2008
Technical
University Munich, Prof. R. Niessner, Munich, Germany, 2008
XIII
International Symposium on Luminescence Spectrometry, Bologna, Italy,
2008
University
of Goettingen, Prof. M. Baehr, Goettingen, Germany, 2008
Protein
aggregation workshop NeuroNE, Soelden, Austria, 2008
Midterm
meeting Nordic Centre
of Excellence, Copenhagen, Denmark 2007
Annual
meeting
NCoE, Karolinska Institute, Stockholm, Sweden 2007
Neurofortis
Conference, Arild, Sweden 2007
Rikshospitalet,
University of Oslo, Norway 2006
Annual
Meeting
Nordic Centre of Excellence, Kuopio, Finland 2006
Karolinska
Institute, Novum, Stockholm, Sweden 2005
Department
of
General Zoology and Neurobiology, Leipzig, Germany 2005
Neurobiology
Research Unit, Copenhagen University Hospital, Copenhagen, Denmark 2004
34th Annual
Meeting, The Society of Neurosciences,
San Diego, USA 2004
GBM-conference
studygroup neurochemistry, Leipzig, Germany 2004
University
of
Texas Medical Branch, Galveston, USA 2004
University
of
Texas Medical Branch, Galveston, USA 2003
GBM-conference
studygroup neurochemistry, Magdeburg, Germany 2002
Poster
presentations:
Author or co-author of
more than 60 conference posters
Miscellanous:
Reviewer for:
Angewandte Chemie Int. Ed.
Journal of the American Chemical Society (JACS)
Analytical Chemistry
Analytical and Bioanalytical
Chemistry
European
Journal of Neurosciences
Chemistry - A European Journal
Journal of Proteomics
Protein Engineering, Design & Selection
and other journals
Coorganization of conferences:
Saxon Biotechnololgy Symposium, Leipzig, 2009 (Final program, pdf)
GBM-conference
Neurochemistry,
Leipzig, 2004
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