. .

Forschung - Research

Group Prof. Dr. D. Huster

Research interests

1. Structural Studies of Fibril Forming Proteins by Solid-State NMR

A disorder in the N-terminal domain of the nuclear poly-(A) binding protein (PABPN1) leads to the conversion of the soluble protein into an insoluble fibrillar form, which is the molecular basis for the Oculopharyngeal Muscular Dystrophy (OPMD). This conversion is caused by a trinucleotide expansion leading to the extension of the Ala stretch (with 10 Ala for the wild type) to maximal 17 Ala residues.

The goal of this project is to gain understanding of the fibrillar structure and formation by solid-state NMR. Therefore the 13C isotropic chemical shifts and torsion angles of the (selec-tive) isotopic labeled N-terminus of PABPN1 will be investigated.

Cooperation:
  • PD Dr. Elisabeth Schwarz, Institute of Biotechnology, Martin-Luther-University Halle-Wittenberg

2. Solid-State NMR Investigations of Membrane Bound Peptides

Secondary structure, membrane interaction, and membrane topology of the putative mem-brane anchor of the protein twisted dwarf 1 (TWD1) from Arabidopsis thaliana are investi-gated by different solid-state NMR techniques. To this end, an isotopically labeled peptide is incorporated into multilamellar vesicles. By static 31P- and 2H-NMR the membrane interac-tion of the peptide is revealed, 13C-MAS-NMR elucidate the secondary peptide structure, while 15N NMR on oriented lipid membranes is used to gain insight into the membrane orien-tation of the TWD1 peptide. We conclude that the membrane anchor of TWD1 consists of an amphipathic alpha-helix located in the membrane interface.

Cooperation:
  • PD Dr. Bernd W. Koenig, Forschungszentrum Jülich
Publication:
  • Holger A. Scheidt, A. Vogel, A. Eckhoff, B. W. Koenig, D. Huster: "Solid-state NMR characterization of the putative membrane anchor of TWD1 from Arabidopsis thaliana". European Biophysical Journal 36 (2007) 393-404

3. Synthesis and folding of NPY-receptors for structural studies

G protein-coupled receptors (GPCRs) are integral membrane proteins involved in a number of signal transduction pathways triggered by different types of ligands. The understanding of this ligand-binding may contribute to the successful production of receptor-active drugs and is therefore of high interest for pharmaceutical research. Currently structural data are only for a single member, bovine rhodopsin, available. This project focuses on the class A GPCRs NPY-receptors expressed in E.coli.

Cooperation:
  • Prof. Dr. Annette G. Beck-Sickinger, Insitute for Biochemistry, University of Leipzig
  • Prof. Dr. Rainer Rudolph, Institute for Biotechnology, University of Halle-Wittenberg

4. Study of the structure and dynamics of the membrane-associated human signal protein N-Ras

Fig. 4: Structural model of the membrane-associated human N-Ras protein with lipidmodified C-terminus.

Cooperation:
  • Prof. Dr. Herbert Waldmann, PD Dr. Jürgen Kuhlmann, Max Planck Institute of Molecular Physiology, Dortmund
Publications:
  • Guido Reuther, Kui-Thong Tan, Julia Köhler, Christine Nowak, André Pampel, Klaus Arnold, Jürgen Kuhlmann, Herbert Waldmann and Daniel Huster: "Structural model of the membrane bound C-terminus of lipid-modified human N-Ras protein". Angew. Chemie 118 (2006) 5387-5390; Angew. Chemie Int. Ed. 45 (2006) 5513-5517
  • Guido Reuther, Kui-Thong Tan, Alexander Vogel, Christine Nowak, Klaus Arnold, Jürgen Kuhlmann, Herbert Waldmann, and Daniel Huster: "The lipidated membrane anchor of full length N-Ras protein shows an extensive dynamics as revealed by solid-state NMR spectroscopy" J. Am. Chem. Soc. 128 (2006) 13840-13846

5. Characterisation of the material properties of articular and artificial cartilage tissue

Fig. 5: Schematic representation of the molecular organization of macromolecules in articular cartilage (green: collagen, red: hyaluronan, black: core protein, blue: chondroitin sulfate and keratan sulfate). Rigid collagen fibrils and highly mobile proteoglycan molecules make up for approximately 25 wt% while cartilage consists of about 75 wt% water.

Cartilage is a complicated gel-like tissue formed of cross-linked collagen, proteoglycane, and water (Fig. 5). The cartilage cells (chondrocytes) only make up for ~1 vol.% of the tissue. We study the dynamical properties of the macromolecules in cartilage tissue in the time window from pico- to milliseconds to understand the viscoelastic properties of the tissue using CP based techniques such as DIPSHIFT and Lee Goldburg CP.

Modern cell techniques allow to produce tissue engineered cartilage for a regenerative therapy. With our methods, we investigate this artificial cartilage tissue to carry out a quality control (QC) quality assurance (QA) analysis.

Cooperation:
  • Prof. Dr. Klaus Arnold, PD Dr. Jürgen Schiller, Dr. André Pampel, University of Leipzig
  • Prof. Dr. Augustinus Bader, Ronny Schulz, Biotechnological-biomedical Center of the University of Leipzig
  • PD Dr. D. Reichert, Martin-Luther-Universität Halle-Wittenberg
  • Prof. Dr. T. J. Bonagamba, Prof. Dr. E. A. deAzevedo, Instituto de Física de São Carlos, Universidade São Paulo, Brazil
  • Bruker BioSpin, Karlsruhe
Publications:
  • Göran Zernia and Daniel Huster: "Collagen dynamics in articular cartilage under osmotic pressure" NMR Biomed. 19 (20 06) 1010-1019
  • Daniel Huster: "Solid-State NMR Studies of Collagen Structure and Dynamics in Isolated Fibrils and in Biological Tissues" Ann. Rep. N MR Spectrosc. (2007) in press

6. Design of lipophilic nucleosides for the nanostructuring and functionalization of biomembranes

Fig. 6: Principle of the functionalization of membrane surfaces by lipophilic nucleosides. Single stranded nucleic acid sequences can recognize the patterns of the membrane anchored nucleosides and bind by Watson Crick base pairing. Thus, enzymes, probes, electrically conductive building blocks, pharmaceuticals and so on can be specifically bound to the membrane surface.

The potential of newly synthesized amphiphilic hybrid molecules for nanotechnological applications is investigated. These lipophilic molecules are inserted into a lipid membrane exposing a nucleobase moiety to the aqueous phase. These molecules may be useful building blocks to obtain functionalized membrane surface that can be recognized by complementary nucleic acid strands. Single stranded DNA or RNA molecules are supposed to bind to the membrane associated structures. Thus, functional molecules can be brought to the membrane surface in a highly specific fashion (Fig. 6). We investigate the localization of the nucleobase in the membrane and the base pairing between complementary molecules by 1H MAS NMR spectroscopy.

Cooperation:
  • Prof. Dr. Jürgen Liebscher, Prof. Dr. Andreas Herrmann, Humboldt University Berlin
Publications:
  • Andreas Bunge, Anke Kurz, Anne-Katrin Windeck, Maximilian Rost, Wolfgang Flasche, Jürgen Liebscher, Andreas Herrmann, Daniel Huster "Lipophilic oligonucleotides spontaneously insert into lipid membranes, bind complementary DNA strands, and translocate into lipid disordered domains" Langmuir 2 3 (2007) 4455-4464

7. NMR techniques

We are also working on the development of solid-state NMR techniques to study the structure and dynamics of proteins and other biological macromolecules in their natural environment.

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Projects

Prokaryontische Expression und strukturelle Charakterisierung von G-Protein gekoppelten Rezeptoren

Im Projekt sollen erstmalig Struktur und Dynamik von rezeptorgebundenem Neuropeptid Y (NPY) aufgeklärt und die Bindungsstelle an seinem G-Protein-gekoppelten Y-Rezeptor bestimmt werden. Dazu werden wir moderne Verfahren der rekombinanten Proteinsynthese benutzen, um ausreichende Mengen an Rezeptor herzustellen, die anschließend in planare Lipidmembranen rekonstituiert werden. Für die Strukturuntersuchungen wird vorrangig die Festkörper-NMR-Spektroskopie eingesetzt, wobei wir in den Experimenten selektiv isotopenmarkiertes NPY und isotopenmarkierten Y-Rezeptor verwenden.

Analytik der extrazellulären Matrix in künstlichem Knorpelgewebe mittels NMR-Spektroskopieund MALDI-TOF-Massenspektrometrie

Trotz ihrer extremen Verbreitung und den daraus resultierenden gewaltigen Folgekosten sind die Ansätze zur Therapie rheumatischer, traumatischer und degenerativer Knorpelerkrankungen bislang nur wenig erfolgreich. Im vorliegenden Projekt sollen Methoden des Tissue Engineerings eingesetzt werden, um künstliches Knorpelgewebe für Gewebetransplantationen herzustellen. Dazu werden Knorpelzellen in verschiedene Trägermatrizes eingesetzt und in einem Bioreaktor kultiviert. Ein wesentliches Problem bei der Gewebeherstellung liegt darin, dass Methoden zur schnellen und zuverlässigen Qualitätskontrolle bislang nicht zur Verfügung stehen. Deshalb werden wir neben den etablierten Referenzverfahren (z.B. (immun)histochemische Verfahren) neue 13C-NMR- und massenspektrometrische Methoden einsetzen. Dabei sollen Verfahren zum Monitoring des Aufbaus der extrazellulären Matrix, insbesondere von Kollagen und Chondroitinsulfat, entwickelt werden. Die hier vorgeschlagene NMR-Methodik erlaubt erstmalig eine quantitative Kontrolle der Qualität des künstlichen Gewebes, ohne dass invasive Vorbehandlungen durchgeführt werden müssen. Dazu werden wir einen speziellen NMR-Probenkopf entwickeln, mit dem die online-Qualitätskontrolle des Gewebes möglich ist, ohne den sterilen Zellkulturkreislauf zu unterbrechen. Durch die innovative Kombination der unterschiedlichen Expertisen der Antragsteller soll im Projekt erstmalig eine umfassende Analytik von künstlichem Knorpelgewebe mit NMR- und Massenspektrometrie durchgeführt werden.

Knorpelregeneration aus körpereigenen Stammzellen

Das Regenerationsvermögen von Knorpel ist relativ gering. Es liegt deshalb nahe mesenchymale Stammzellen (MSC) einzusetzen und diese durch geeignete Maßnahmen zu Knorpelzellen (Chondrozyten) zu differenzieren. Im Projekt wurde bislang primär die Lipid-Zusammensetzung der Zellen in Abhängigkeit von den Kulturbedingungen mittels MALDI-TOF MS wie auch 31P NMR-Spektroskopie untersucht. Dabei zeigten sich typische Veränderungen in Abhängigkeit von den Kulturbedingungen. Darüber hinaus war auch die Unterscheidung normaler und apoptotischer Zellen möglich. In Kooperation mit Bruker Daltonics (Bremen) gelang außerdem die Einwicklung eines speziellen "Adaptertargets" sowie einer entsprechenden Analyse-Software, wodurch das direkte "Abrastern" von TLC-Platten möglich wird. Erste Ergebnisse zur Anwendbarkeit dieser Technik auf medizinisch relevante Lipidgemische sowie Stammzell-Extrakte wurden bereits publiziert.

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Curriculum vitae D. Huster

  • since 10/08: Head of the Institute of Medical Physics and Biophysics, University of Leipzig, Germany (Full professorship for Medical Biophysics)
  • 1/06-9/08: Head of Junior Research Group Structural Biology of Membrane Proteins', Institute of Biotechnology, Martin Luther University Halle-Wittenberg, Germany
  • 9/2001-12/2005: Head of Junior Research Group 'Solid-state NMR studies of membrane-associated proteins', Biotechnological-Biomedical Center, University of Leipzig, Germany
  • 12/99-8/01: Post doctoral fellow, Department of Chemistry, Iowa State University, Ames, IA, USA
  • 7/99-11/99: Post doctoral fellow, Institute of Medical Physics and Biophysics, University of Leipzig, Germany
  • 4/96-6/99: Graduate student, Institute of Medical Physics and Biophysics, University of Leipzig, Germany and Laboratory of Membrane Biochemistry and Biophysics, NIAAA, National Institutes of Health, Rockville, USA. Ph.D. Thesis: "NMR-Untersuchungen zur Wechselwirkung von biologisch relevanten Polyelektrolyten mit Lipidmembranen und Lipoproteinen", University of Leipzig, 1999
  • 9/94-2/96: Diploma thesis, Institute of Medical Physics and Biophysics, University of Leipzig, Germany and Laboratory of Membrane Biochemistry and Biophysics, NIAAA, National Institutes of Health, Bethedsa, USA. Diploma thesis: "NMR-Untersuchungen zur Wasserpermeation von Phospholipidbilayern" University of Leipzig, 1996
  • 10/90-8/94: Undergraduate student, University of Leipzig

Full CV

 Full CV (213 KB; 10.12.2008)

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Group members

NameTitelRaumTelefone-mail

Head

Daniel Huster   Prof. Dr. rer. nat. habil. 148 97 15700 daniel.huster@medizin.uni-leipzig.de

Group members

Christian Berger   Dipl.-Ing. 132 97 15740 Christian.Berger@medizin.uni-leipzig.de
Sandra Berndt   Dipl.-Biochem. 134 97 15742 Sandra.Berndt@medizin.uni-leipzig.de
Julia Böhme   cand. med. 355 97 15773 Julia.Boehme@medizin.uni-leipzig.de
David Foese   cand. phys. 136 97 15744 David.Foese@medizin.uni-leipzig.de
Daniel Huster   Prof. Dr. rer. nat. habil. 148 97 15700 daniel.huster@medizin.uni-leipzig.de
Mario Keller   Dipl.-Ing. (FH) 137 97 15706 mario.keller@medizin.uni-leipzig.de
Ina Khalaf   cand. med. 355.4 97 15773 ina.khalaf@medizin.uni-leipzig.de
Franziska Löbel  
Cindy Montag   TA 134 97 15742 Cindy.Montag@medizin.uni-leipzig.de
Matthias Müller   Dr. rer. nat. 152.1  97 15712  m.mueller@medizin.uni-leipzig.de
Anja Penk   cand. phys. 137 97 15706 Anja.Penk@medizin.uni-leipzig.de
Paula Pescador   Dr. rer. nat. 131 97 15726 Paula.Pescador@medizin.uni-leipzig.de
Thomas Riemer   Dr. rer. nat. 350 97 15768 riemer@rz.uni-leipzig.de
Franziska Salzbrenner   cand. med. 355 97 15773 Franziska.Salzbrenner@medizin.uni-leipzig.de
Holger Scheidt   Dr. rer. nat. 131 97 15726 Holger.Scheidt@medizin.uni-leipzig.de
Peter  Schmidt   Dipl.-Ing. 134 97 15742 Peter.Schmidt@medizin.uni-leipzig.de
Stephan Theisgen   Dipl.-Biochem. 132 97 15740 Stephan.Theisgen@medizin.uni-leipzig.de
Lars Thomas   Dr. rer. nat. 136 97 15744 Lars.Thomas@medizin.uni-leipzig.de
Heiko Weigelt   Dipl.-Phys. 136 97 15744 heiko.weigelt@medizin.uni-leipzig.de
Leif Zernia   cand. med. 355 leif.zernia@medizin.uni-leipzig.de
Peter  Schmidt   Dipl.-Ing. 134 97 15742 Peter.Schmidt@medizin.uni-leipzig.de

Former group members

NameTitelnowe-mail
Patrick  Barré   Dr. rer. nat. Cornell University, New York, USA pmb2005@med.cornell.edu
Andreas Bunge   Dr. rer. nat. Berlin
Dirk Enderlein   Dipl.-Biol. Uni Leipzig, Veterinärmedizinische Fakultät  enderlein@vetmed.uni-leipzig.de
Irina Hirsch   Dr. rer. nat. Max-Planck-Forschungsstelle für Enzymologie der Proteinfaltung, Halle/Saale
Stephanie Höhle   Dr. med.
Guido Reuther   Dr. rer. nat. Albert Einstein College of Medicine New York City, USA
Jessica Schulz   Dr. rer. nat. Deutsches Krebsforschungszentrum Heidelberg j.schulz@dkfz.de
Alexander Vogel   Dr. rer. nat. Martin Luther University Halle Wittenberg, Institute for Biotechnology, Junior research group "Structural Biology of Membrane Proteins"
Kerstin  Wagner   Frau Dr. rer. nat.  MPI für Kolloid- und Grenzflächenforschung Potsdam-Golm kerstin.wagner@mpikg-golm.mpg.de
Göran Zernia   Dr. med.

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Publications

Journal Articles

75. Gaetano Angelici, Nicola Castellucci, Giuseppe Falini, Daniel Huster, Magda Monari, Claudia Tomasini
"Pseudopeptides designed to form supramolecular helices: the role of the stereogenic centers"
Cryst. Growth Des. 10 (2010) 923–929

74. Alexander Vogel, Guido Reuther, Matthew B. Roark, Kui-Thong Tan, Herbert Waldmann, Scott E. Feller, Daniel Huster
"Backbone Conformational Flexibility of the Lipid Modified Membrane Anchor of the Human N-Ras Protein Investigated by Solid-State NMR and Molecular Dynamics Simulation"
Biochim. Biophys. Acta 1798 (2010) 275-285

73. Stephan Theisgen, Holger A. Scheidt, Alvicler Magalhães, Tito J Bonagamba, Daniel Huster
"A Solid-State NMR Study of the Structure and Dynamics of the Myristoylated N-Terminus of the Guanylate–Cyclase Activating Protein–2"
Biochim Biophys Acta - Biomembranes 1798 (2010) 266–274

72. Andreas Bunge, Martin Loew, Paula Pescador, Anna Arbuzova, Nicolai Brodersen, Jing Kang, Lars Dähne, Jürgen Liebscher, Andreas Herrmann, Gudrun Stengel, Daniel Huster
"Functionalization of Lipid Membranes with Lipophilic Oligonucleotides on Layer-by-Layer Coated Particles"
J. Phys. Chem. B 113 (2009) 16425-16434

71. Peter Schmidt, Diana Lindner, Cindy Montag, Sandra Berndt, Annette G. Beck-Sickinger, Rainer Rudolph, Daniel Huster
"Prokaryotic Expression, in vitro Folding, and Molecular Pharmacological Characterization of the Neuropeptide Y Receptor Type 2"
Biotechnol. Progr. 25 (2009) 1732-1739

70. Alexander Vogel, Guido Reuther, Katrin Weise, Gemma Triola, Jörg Nikolaus, Kui-Thong Tan, Christine Nowak, Andreas Herrmann, Herbert Waldmann, Roland Winter, Daniel Huster
"Lipid Modifications of Ras Sense the Membrane Environment and Induce Local Enrichment"
Angew. Chem. Int. Ed. 48 (2009) 8784-8787

69. Andreas Bunge, Martin Fischlechner, Martin Loew, Anna Arbuzova, Andreas Herrmann, Daniel Huster
"Characterization of Lipid Bilayers Adsorbed on Spherical LbL-Support"
Soft Matter. 5 (2009) 3331-3339

68. Gaetano Angelici, Giuseppe Falini, Hans-Jörg Hofmann, Daniel Huster, Magda Monari, Claudia Tomasini
"Nanofibers from Oxazolidi-2-one Containing Hybrid Foldamers: What is the Right Molecular Size?"
Chem. Eur. J. 15 (2009) 8037-8048

67. Lars Thomas, Holger A. Scheidt, Andrea Bettio, Annette G. Beck-Sickinger, Daniel Huster, Olaf Zschörnig
"Membrane Interaction of NPY in the Presence of Negatively Charged and Zwitterionic Phospholipids"
Eur. Biophys. J. 38 (2009) 663-677

66. Holger A. Scheidt, Daniel Huster
"Structure and Dynamics of the Myristoyl Lipid Modification of a Src Peptide Determined by 2H Solid-State NMR Spectroscopy"
Biophys. J. 96 (2009) 3663-3672

65. Anja Hagenau, Holger A. Scheidt, Louise Serpell, Daniel Huster, Thomas Scheibel
"Structural Analysis of Proteinaceous Components in Byssal Threads of the Mussel Mytilus galloprovincialis"
Macromol. Biosci. 9 (2009) 162-168

64. Martin Loew, Jing Kang, Lars Dähne, Ruth Hendus-Altenburger, Oliver Kaczmarek, Jürgen Liebscher, Daniel Huster, Kai Ludwig, Christoph Böttcher, Andreas Herrmann, Anna Arbuzova
"Controlled assembly of vesicle-based nanocontainers on Layer-by-Layer particles via DNA hybridisation"
Small 5 (2009) 320-323

63. Andreas Bunge, Anne-Katrin Windeck, Thomas Pomorski, Jürgen Schiller, Andreas Herrmann, Daniel Huster, Peter Müller
"Biophysical Characterization of a New Phospholipid Analogue with a Spin-labeled Unsaturated Fatty Acid Chain"
Biophys. J. 96 (2009) 1008-1015

62. Luc Brunsveld, Herbert Waldmann, Daniel Huster
"Membrane Binding of Lipidated Ras Peptides and Proteins – the Structural Point of View."
Biochim Biophys Acta - Biomembranes. 1788 (2009) 273-288

61. Gaetano Angelici, Giuseppe Falini, Hans-Jörg Hofmann, Daniel Huster, Magda Monari
"A Fiberlike Peptide Material Stabilized by Single Intermolecular Hydrogen Bonds"
Angew. Chem. Int. Ed. 47 (2008) 8075 - 8078

60. Anja Hagenau, Holger A. Scheidt, Louise Serpell, Daniel Huster, Thomas Scheibel
"Structural Analysis of Proteinaceous Components in Mussel Byssal Threads of the Mussel Mytilus galloprovincialis"
Macromol. Biosci., (2008) in press

59. Andreas Bunge, Peter Müller, Martin Stöckl, Andreas Herrmann, Daniel Huster
"Characterization of the ternary mixture of sphingomyelin, POPC, and cholesterol: Support for an inhomogeneous lipid distribution at high temperatures"
Biophys. J. 94 (2008) 2680-2690

58. Astrid Vieler, Holger A. Scheidt, Peter Schmidt, Cindy Montag, Janine F. Nowoisky, Martin Lohr, Christian Wilhelm, Daniel Huster and Reimund Goss
"The influence of phase transitions in phosphatidylethanolamine models on the activity of violaxanthin de-epoxidase"
Biochimica et Biophysica Acta (BBA) - Biomembranes 1778 (2008) 1027-1034

57. Daniel Huster
"Solid-State NMR Studies of Collagen Structure and Dynamics in Isolated Fibrils and in Biological Tissues"
Ann. Rep. NMR Spectrosc. (2007) in press

56. Alexander Vogel, Thomas Schröder, Christian Lange, and Daniel Huster
"Characterization of the Myristoyl Lipid Modification of Membrane Bound GCAP-2 by 2H Solid-State NMR Spectroscopy"
Biochim. Biophys. Acta 1768 (2007) 3171-3181  

55. Holger A. Scheidt and Daniel Huster
"The Interaction of Small Molecules with Phospholipid Membranes studied by 1H NOESY NMR under Magic-Angle Spinning"
Acta Pharmacol. Sin. 29 (2008) 35-49

54. Nicolai Brodersen, Jun Li, Oliver Kaczmarek, Andreas Bunge, Ludwig Löser, Daniel Huster, Andreas Herrmann, Jürgen Liebscher
"Nucleosides with 5´-Fixed Lipid Groups – Synthesis and Anchoring in Lipid Membranes"
Eur. J. Org. Chem. 36 (2007) 6060-6069

53. Alexander Vogel, Kui-Thong Tan, Herbert Waldmann, Scott E. Feller, Michael F. Brown, and Daniel Huster
"Flexibility of Ras Lipid Modifications Studied by 2H Solid-State NMR and Molecular Dynamics Simulations"
Biophys. J. 93 (2007) 2697-2712

52. Marcus Haberhauer, Göran Zernia, Ronny Schulz, Andrea Deiwick, Claudia Schnepp, Daniel Huster, and Augustinus Bader
"Tissue engineered cartilage constructs grown in allogenous plasma and whole blood nanoscaffolds"
Adv. Mater. (2007) in press

51. Jürgen Schiller, Matthias Müller, Beate Fuchs, Klaus Arnold, Daniel Huster
"31P NMR spectroscopy of phospholipids. From micelles to membranes"
Current Analytical Chemistry 3 (2007) 283-301

50. Jessica Schulz, Mathias Pretzsch, Ina Khalaf, Andrea Deiwick, Holger A. Scheidt, Georg von Salis-Soglio, Augustinus Bader, and Daniel Huster
"Quantitative monitoring of extracellular matrix production in bone implants by 13C and 31P solid-state NMR spectroscopy"
Calcif. Tissue Int. 80 (2007) 275-285

49. Frank Bringezu, Monika Majerowicz, Shaoying Wen, Guido Reuther, Kui-Thong Tan, Juergen Kuhlmann, Herbert Waldmann, and Daniel Huster
"Membrane binding of a lipidated N-Ras protein studied in lipid monolayers"
Eur. Biophys. J. 36 (2007) 491-498

48. Holger A. Scheidt, Alexander Vogel, Andreas Eckhoff, Bernd W. Koenig, and Daniel Huster
"Solid-state NMR characterization of the putative membrane anchor of TWD1 from arabidopsis thaliana"
Eur. Biophys. J. 36 (2007) 393-404

47. Andreas Bunge, Anke Kurz, Anne-Katrin Windeck, Maximilian Rost, Wolfgang Flasche, Jürgen Liebscher, Andreas Herrmann, Daniel Huster
"Lipophilic oligonucleotides spontaneously insert into lipid membranes, bind complementary DNA strands, and translocate into lipid
disordered domains"         
Langmuir 23 (2007) 4455-4464

46. Guido Reuther, Kui-Thong Tan, Alexander Vogel, Christine Nowak, Klaus Arnold, Jürgen Kuhlmann, Herbert Waldmann, and Daniel Huster
"The  lipidated membrane anchor of full length N-Ras protein shows an extensive dynamics as revealed by solid-state NMR spectroscopy"
J. Am. Chem. Soc. 128 (2006) 13840-13846

45. Anke Kurz, Andreas Bunge, Anne-Katrin Windeck, Maximilian Rost, Wolfgang Flasche, Anna Arbuzova, Denise Strobach,
Sabine Müller, Jürgen Liebscher, Daniel Huster, Andreas Herrmann
"Lipid-anchored oligonucleotides for stable double helix formation in distinct membrane domains"
Angew. Chemie 118 (2006) 4550-4554; Angew. Chemie Int. Ed. 45 (2006) 4440-4444

44. Guido Reuther, Kui-Thong Tan, Julia Köhler, Christine Nowak, André Pampel, Klaus Arnold, Jürgen Kuhlmann,
Herbert Waldmann and Daniel Huster
"Structural model of the membrane bound C-terminus of lipid-modified human N-Ras protein"
Angew. Chemie 118 (2006) 5387-5390; Angew. Chemie Int. Ed. 45 (2006) 5513-5517

43. Göran Zernia and Daniel Huster
"Collagen dynamics in articular cartilage under osmotic pressure"
NMR Biomed. 19 (2006) 1010-1019

42. Ronny Schulz, Stephanie Höhle, Göran Zernia, Matthias Zscharnack, Jürgen Schiller, Augustinus Bader, and Daniel Huster
"Analysis of extracellular matrix production in artificial cartilage constructs by histology, immunocytochemistry, mass
spectrometry, and NMR spectroscopy"
J. Nanosci. Nanotech. 6 (2006) 2368-2381

41. Toni Vagt, Olaf Zschörnig, Daniel Huster, and Beate Koksch
"Membrane binding an structure of a cationic coiled coil peptide investigated by CD, fluorescence, and solid-state NMR spectroscopy"
ChemPhysChem 7 (2006) 1361-1371

40. Alexander Vogel, Catherine Katzka, Herbert Waldmann, Klaus Arnold, Michael F. Brown, and Daniel Huster
"Lipid modifications of a Ras peptide exhibit altered packing and mobility versus host membrane as detected by 2H solid-state
NMR"
J. Am. Chem. Soc. 127 (2005) 12263-12272

39. Holger A. Scheidt, Daniel Huster, and Klaus Gawrisch
"Diffusion of cholesterol and its precursors in lipid membranes studied by 1H PFG MAS NMR"
Biophys. J. 89 (2005) 2504-2512

38. Lars Thomas, Holger A. Scheidt, Andrea Bettio, Daniel Huster, Annette Beck-Sickinger, Klaus Arnold, and Olaf Zschörnig
"Neuropeptide Y - membrane interaction detected by EPR and NMR spectroscopy"
Biochim. Biophys. Acta 1714 (2005) 103-113

37. Daniel Huster
"Investigations of the structure and dynamics of membrane-associated peptides by magic angle spinning NMR"
Prog. Nucl. Magn. Reson. Spectrosc. 46 (2005) 79-107

36. Daniel Huster, Holger A. Scheidt, Klaus Arnold, Andreas Herrmann and Peter Müller
"Desmosterol may replace cholesterol in biological membranes"
Biophys. J. 88 (2005) 1838-1844

35. Holger A. Scheidt, Wolfgang Flasche, Crina Cismas, Maximilian Rost, Andreas Herrmann, Jürgen Liebscher, and Daniel Huster
"Design and application of lipophilic nucleosides as building blocks to obtain highly functional biological surfaces"
J. Phys. Chem. B 108 (2004) 16279-16287

34. Kerstin Wagner, A. S. Beck-Sickinger, and Daniel Huster
"Structural investigations of a human calcitonin-derived carrier peptide in membrane environment by solid-state NMR"
Biochemistry 43 (2004) 12459-12468

33. Daniel Huster, Lama Naji, Jürgen Schiller, and Klaus Arnold
"Molecular dynamics of the biopolymers in articular cartilage studied by magic angle spinning NMR"
Appl. Magn. Reson. 27 (2004) 471-487

32. Holger A. Scheidt, André Pampel, Ludwig Nissler, Rolf Gebhardt, and Daniel Huster
"Investigation of the membrane localization and dynamics of flavonoids by high-resolution magic angle spinning NMR spectroscopy"
Biochim. Biophys. Acta 1663 (2004) 97-107

31. Detlef Reichert, Ovidiu Pascui, Eduardo R. deAzevedo, Tito J. Bonagamba, Klaus Arnold, and Daniel Huster
"A solid-state NMR study of the fast and slow dynamics of collagen fibrils at varying hydration"
Magn. Reson. Chem. 42 (2004) 276-284

30. Holger Scheidt, Peter Müller, Andreas Herrmann, and Daniel Huster
"The potential of fluorescent and spin labeled steroid analogs to mimic natural cholesterol"
J. Biol. Chem. 278 (2003) 45563-54569

29. Patrick Barré, Olaf Zschörnig, Klaus Arnold, and Daniel Huster
"Structural and dynamical changes of the bindin B18 peptide upon binding to lipid membranes. A solid-state NMR study"
Biochemistry 42 (2003) 8377-8386

28. Patrick Barré, Satoru Yamaguchi, Daniel Huster, and Hazime Saito
"Backbone dynamics of bacteriorhodopsin as studied by 13C solid-state NMR spectroscopy"
Eur. Biophys. J. 32 (2003) 578-584

27. Daniel Huster, Alexander Vogel, Catherine Katzka, Holger A. Scheidt, Hans Binder, Olaf Zschörnig, Silvia Dante,
Thomas Gutberlet, Herbert Waldmann, and Klaus Arnold
"Membrane insertion of a lipidated ras peptide by FTIR, solid-state NMR, and Neutron diffraction spectroscopy"
J. Am. Chem. Soc. 125 (2003) 4070-4079

26. Alexander Vogel, Holger A. Scheidt, and Daniel Huster
"The distribution of lipid attached EPR probes in bilayers. Application to membrane protein topology"
Biophys. J. 85 (2003) 1691-1701

25. Daniel Huster, Peter Müller, Klaus Arnold, and Andreas Herrmann
"The distribution of chain attached 7-nitrobenz-2oxa-1,3-diazol-4-yl (NBD) in acidic membranes determined by 1H MAS NMR spectroscopy"
Eur. Biophys. J. 32 (2003) 47-54

24. Daniel Huster, Jürgen Schiller, and Klaus Arnold
"Comparison of collagen dynamics in cartilage and isolated fibrils by solid-state NMR spectroscopy"
Magn. Reson. Med. 48 (2002) 624-632

23. Mei Hong, Xiaolan Yao, Karen Jakes, and Daniel Huster
"Investigation of molecular motions by magic-angle cross-polarization NMR spectroscopy"
J. Phys. Chem. B 106 (2002) 7355-7364

22. Daniel Huster, Xiaolan Yao, and Mei Hong
"Membrane protein topology probed by 1H spin diffusion from lipids using solid-state NMR spectroscopy"
J. Am. Chem. Soc. 124 (2002) 874-883

21. Daniel Huster, Xiaolan Yao, Karen Jakes und Mei Hong
"Conformational changes of colicin Ia channel-forming domain upon membrane binding: a solid-state NMR study"
Biochim. Biophys. Acta 1561 (2002) 159-170

20. Satoru Yamaguchi, Daniel Huster, Alan Waring, Robert I. Lehrer, William Kearney, Brian F. Tack, and Mei Hong
"Orientation and dynamics of an antimicrobial peptide in the lipid bilayer by solid-state NMR spectroscopy"
Biophys. J. 81 (2001) 2203-2214

19. Daniel Huster, Linshi Xiao, and Mei Hong
"Solid-state NMR investigation of the dynamics of soluble and membrane-bound colicin Ia channel-forming domain"
Biochemistry 40 (2001) 7662-7674

18. Martin Rödenbeck, Matthias Müller, Daniel Huster, and Klaus Arnold
"Counterion condensation as saturation effect under the influence of ion hydration"
Biophys. Chem. 90 (2001) 255-268

17. Daniel Huster, Peter Müller, Klaus Arnold, and Andreas Herrmann
"Dynamics of membrane penetration of the fluorescent 7-nitrobenz-2-oxa-1,3-diazol-4-yl (NBD) group attached to an acyl chain of
phosphatidylcholine"
Biophys. J. 80 (2001) 822-831

16. Daniel Huster, Karsten Kuhn, Dieter Kadereit, Herbert Waldmann, and Klaus Arnold
"High resolution magic angle spinning NMR for the investigation of a ras lipopeptide in a lipid membrane"
Angew. Chemie 113 (2001) 1083-1085; Angew. Chemie Int. Ed. 40 (2001) 1056-1058

15. Jürgen Schiller, Lama Naji, Daniel Huster, Jörn Kaufmann, and Klaus Arnold
"1H and 13C HR-MAS NMR investigations on native and enzymatically-digested cartilage. Evidence for marked differences
in extraction efficiency"
MAGMA 13 (2001) 19-27

14. Daniel Huster, Satoru Yamaguchi, and Mei Hong
"Efficient b sheet structure determination in proteins by solid state NMR spectroscopy"
J. Am. Chem. Soc. 122 (2000) 11320-11327

13. Daniel Huster, Undine Dietrich, Thomas Gutberlet, Klaus Gawrisch, and Klaus Arnold
"Lipid matrix properties in cationic membranes interacting with anionic polyelectrolytes - a solid state NMR approach"
Langmuir 16 (2000) 9225-9232

12. Klaus Dähnert and Daniel Huster
"Thermodynamics of the laminar Donnan system"
J. Colloid Interface Sci. 228 (2000) 226-237

11. Daniel Huster, Klaus Arnold, and Klaus Gawrisch
"Strength of Ca2+ binding to retinal lipid membranes - consequences for lipid organization"
Biophys. J. 78 (2000) 3011-3018

10. Lama Naji, Jörn Kaufmann, Daniel Huster, Jürgen Schiller, Klaus Arnold
"13C NMR relaxation study on cartilage and cartilage components. The origin of 13C NMR spectra of cartilage"
Carbohyd. Res. 327 (2000) 439-446

9. Scott E. Feller, Daniel Huster, and Klaus Gawrisch
"Interpretation of NOESY cross-relaxation rates from MD simulation of a lipid bilayer"
J. Am. Chem. Soc. 121 (1999) 8963-8964

8. Klaus Dähnert and Daniel Huster
"Comparison of the Poisson-Boltzmann model and the Donnan equilibrium of a polyelectrolyte in salt solution"
J. Colloid Interface Sci. 215 (1999) 131-139

7. Daniel Huster and Klaus Gawrisch
"NOESY NMR crosspeaks between lipid headgroups and hydrocarbon chains - spin diffusion or molecular disorder?"
J. Am. Chem. Soc. 121 (1999) 1992-1993

6. Daniel Huster, Gerrit Paasche, Undine Dietrich, Olaf Zschörnig, Thomas Gutberlet, Klaus Gawrisch, and Klaus Arnold
"Investigation of phospholipid area compression induced by calcium mediated dextran sulfate interaction"
Biophys. J. 77 (1999), 879-887

5. Albert J. Jin, Daniel Huster, Klaus Gawrisch, and Ralph Nossal
"Light scattering characterization of extruded lipid vesicles"
Eur. Biophys. J. 28 (1999) 187-199

4. Daniel Huster, Klaus Arnold, and Klaus Gawrisch
"Investigation of lipid organization in biological membranes by two-dimensional nuclear Overhauser enhancement spectroscopy"
J. Phys. Chem. B 103 (1999) 243-251

3. Daniel Huster and Klaus Arnold
"Ca2+-mediated interaction between dextran sulfate and dimyristoyl-sn-glycero-3-phosphocholine surfaces studied by 2H NMR"
Biophys. J. 75 (1998) 909-916

2. Daniel Huster, Klaus Arnold, and Klaus Gawrisch
"Influence of docosahexaenoic acid and cholesterol on lateral lipid organization in phospholipid membranes"
Biochemistry 37 (1998) 17299-17308

1. Daniel Huster, Albert J. Jin, Klaus Arnold, and Klaus Gawrisch
"Water permeability of polyunsaturated membranes measured by 17O NMR"
Biophys. J. 73 (1997) 855-864

Invited Papers

6.   Göran Zernia and Daniel Huster
"Investigation of tissue collagen dynamics by solid-state NMR spectroscopy" in "Handbook of Modern Magnetic Resonance"
Editor: G. Webb. Kluwer Academic Publisher, 2005, 79-84

5. Daniel Huster, Jürgen Schiller, Lama Naji, Jörn Kaufmann, Klaus Arnold
"NMR studies of cartilage - Dynamics, diffusion and degradation" in "Molecules in interaction with surfaces" Lecture Notes in Physics
Editors: R. Haberland, A. Pöppl, R. Stannarius, and D. Michel. Springer Verlag, Heidelberg, 2004, 455-492

4. Jürgen Schiller, Daniel Huster, Beate Fuchs, Lama Naji, Jörn Kaufmann, and Klaus Arnold
"Evaluation of cartilage composition and degradation by high resolution magic angle spinning nuclear magnetic resonance" in
"Osteoarthritis: Methods and Protocols" Editors: F. De Ceuninck, P. Pastoureau, M. Sabatini. Humana Press, Totowa, 2004, 269-287

3. Daniel Huster, Jürgen Schiller, and Klaus Arnold
"Solid state NMR to study the dynamics of cartilage polymers" in "Osteoarthritis: Methods and Protocols"
Editors: F. De Ceuninck, P. Pastoureau, M. Sabatini. Humana Press, Totowa, 2004, 307-322

2. Jürgen Schiller, Beate Fuchs, Daniel Huster, Lama Naji, and Klaus Arnold
"Structural properties of normal and diseased cartilage from the view of biophysical chemistry" in "Recent research developments in Biochemistry"
Current Topics in Biochemical Research, Vol. 5 Editor: Uma Ramchandran, Research Prints, Trivandrum, 2003, 43-63

1. Daniel Huster and Klaus Gawrisch
"New insights into biomembrane structure from two-dimensional nuclear Overhauser enhancement spectroscopy" in
"Lipid Bilayers Structure and Interactions" Editors: John Katsaras and Thomas Gutberlet, Springer Verlag, Berlin, 2000, 109-125

Dissertations

3.   Daniel Huster
"Festkörper-NMR-Untersuchungen zur Struktur und Dynamik membrangebundener und Fibrillen bildender Proteine"
Habilitation, University of Leipzig, 2004

2. Daniel Huster
"NMR-Untersuchungen zur Wechselwirkung von biologisch relevanten Polyelektrolyten mit Lipidmembranen und Lipoproteinen"
Ph. D. thesis, University of Leipzig, 1999

1. Daniel Huster
"NMR-Untersuchungen zur Wasserpermeation von Phospholipidbilayern"
Diploma thesis, University of Leipzig, 1996