D = Dermatological Clinic, U = Urological Clinic, *G= in cooperation with the Department of Obstetrics and Gynaecology, *in cooperation with the Department of Clinical Chemistry and Pathological Biochemistry, ** in cooperation with the Department of Internal Medicine III, # in cooperation with the Department of Medical Microbiology, *P= in cooperation with the Department of Pathology

1. Ultrasound(U)

• Doppler ultrasonography (bidirectional, 4 Mhz/8Mhz), Duplex ultrasonography, Colour-duplex ultrasonography, Ultrasonography of the testes, Transrectale ultrasonography of the prostate and seminal glands

Ultrasonographic guided punctions (prostate, seminal glands), Ultrasonography of the kidneys and abdominal organs

2. Semen delivery (D/U)

• Split-ejaculates, Pooled-ejaculates, Gutron-Test

3. Classical spermiogram (D)

• Spermiogram according to the WHO-guidelines (including the detection of peroxidase-positive cells)
• Computer aided sperm motion analysis, CASA (Stroemberg-Mika-System)
• Detection of the motility after 4 and/or 24 hours
• Evaluation of spermatozoal morphology (Shorr-staining, ultramicroscopic technique *Pathol. Institute)
• Morphometry
• Hypo-osmotic swelling test

4. Biochemical analyses (D)

• Fructose
• alpha-Glucosidase *
• Elastase *
• Prostata-spezific antigen (PSA) in seminal plasma and in serum*
• IgA, IgG, C 3 complement, coeruloplasmin*

5. Immunological evaluations (D)

• Antisperm antibodies in seminal plasma (Agglutination-test)
• Sperm bound antibodies (mixed antiglobulin reaction test, MAR-test)
• Antisperm antibodies in serum
• Antisperm antibodies in cervical mucus
• Immunocytochemistry

6. Sperm-cervical mucus-interaction (D /*G)

• Spermatozoa-cervix mucus-contact test (SCMC)
• Postcoital-test according to Sims-Huhner
• Kurzrok-Miller-test (Slide-test)
• Penetrak-test

7. Sperm function tests (D)

• Cryopreservation of semen
• Acrosin activity (Gelatinolyse/enzymatical)
• Acrosome reaction (spontan/induziert)
• Nuclear chromatin condensation (Anilinblue-staining)
• Evaluation of the freezability of human spermatozoa
• Stimulation of the sperm motility (Pentoxifyllin, Kallikrein)
• Integrity of the sperm double stranded DNA (Acridine orange-Staining)
• Detection of deoxyribose
• Flow cytometric analyses of spermatozoa

8. Preparation techniques of spermatozoa for assisted fertilization (D)

• Swim-up
• Glaswool filtration
• Percoll density gradient centrifugation (including minipercoll-technique)
• Migration-sedimentation-technique
• Magnetic activated cell sorting (MACS)

9. Assisted fertilization (*G):

• Intrauterine insemination of separated spermatozoa(IUI)
• In-vitro-Fertilization (IvF)
• Intracytoplasmatic sperm injection (ICSI)

10. Urine analysis (D/U)

• Sediment of urine after ejaculation
• Urine analysis
• Analysis of prostate fluid
• Analysis of urethral discharge

11. Endokrinological analysis and tumor marker

• FSH*/**, LH*/**, Testosterone*/**, Free testosterone*/**
• Prolactin*/**, Estradiol*/**
• Sexual hormone binding globulin (SHBG)*
• Androstendione*
• DHEA-Sulfate*/**
• Progesterone*, 17-OH-Progesterone*
• Cortisole*/**
• Human growth hormone (hGH)*
• ß-HCG*
• AFP*
• PSA*

12. Hormonal stimulation tests (D)

• GnRH-test, HCG-test

13. Microbiological analysis

• Cultural detection of Gonococci (D)
• Classification of mycoplasmas (#)
• Quantitative detection of mycoplasmas (#)
• IgA/IgG-antibodies against Chlamydia in seminal plasma (#)
• Detection of Chlamydia (#)
• Mycological and bacteriological semen culture (D)
• Ultrasonographical guided punction of the prostate and the seminal glands (U)

14. Testicular biopsy

• Andrological operation (U)
• Histological examination of the tissue (D,*P)
• Semithin section-technique (*P,D)
• Ultramicroscopy (*P)
• Enzymatic histochemistry (D/*P)
• Immunohistochemistry (D/*P)

15. Function tests in erectile dysfunction (U)

• Doppler examination of the penile arteria before and after injection of vasoactive agents
• Intracavernous injection testing with vasoactive drugs (PgE 1, „Triple Drug“)
• Duplex ultrasonography of the penile arteries
• „Colour“-duplex ultrasonography of the penile arteries
• Three dimensional Doppler assessment during artificial erection
• Diagnostic vacuum-erection
• Dynamic Pharmaco-Cavernosometry and Cavernosography (DICC)
• Artificial erection
• Angiography of the penile arteria during artificial erection

16. Microsurgery (U) (MESA, TESE, VVST, VEST)

• Operative microscope
• Completely microsurgical equipment

17. Artificial ejaculation

• Vibration ejaculator