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Publications: Textbook Contributions - Soft Matter Physics Division - University of Leipzig
Publications Textbook
Contributions
| Carsten Stüber, Tobias Kießling, Anatol
Fritsch, Franziska Wetzel, Christian Schulze, Dan Strehle, Josef Käs: |
| Optical
Cell Manipulation |
 Abstract
of Chapter 36: In current biotechnology and biomedicine, cell-based
investigations are rapidly gaining importance due to the recognition that
molecular research alone does not provide a sufficiently complex insight.
The high cost of animal studies and the fact that their results often cannot
be directly transferred to humans additionally shifts the focus towards
cell-based assays. This current trend triggers the development of more
sophisticated laser-based techniques to manipulate and investigate cells.
In this rapidly changing field, it is nearly impossible to provide a comprehensive
view of all available techniques, since new techniques are constantly developed
on nearly a weekly basis. In this chapter, we have made an effort to provide
the physical basis for laser-based cell manipulation, complemented by several
of the more exceptional applications of optical cell manipulation.
Book Description: Since 2004 and with
the 2nd edition in 2007, the Springer Handbook of Nanotechnology has established
itself as the definitive reference in the nanoscience and nanotechnology
area. It integrates the knowledge from nanofabrication, nanodevices, nanomechanics,
Nanotribology, materials science, and reliability engineering in just one
volume. Beside the presentation of nanostructures, micro/nanofabrication,
and micro/nanodevices, special emphasis is on scanning probe microscopy,
nanotribology and nanomechanics, molecularly thick films, industrial applications
and microdevice reliability, and on social aspects. In its 3rd edition,
the book grew from 8 to 9 parts now including a part with chapters on biomimetics.
More information is added to such fields as bionanotechnology, nanorobotics,
and (bio)MEMS/NEMS, bio/nanotribology and bio/nanomechanics. The book is
organized by an experienced editor with a universal knowledge and written
by an international team of over 150 distinguished experts. It addresses
mechanical and electrical engineers, materials scientists, physicists and
chemists who work either in the nano area or in a field that is or will
be influenced by this new key technology.
|
| Bharat Bhushan (Ed.): Springer Handbook of Nanotechnology,
3rd Edition, Chapter 36 (1201-1226), Springer, Berlin Heidelberg (2010) |
| Karla Müller, Anatol Fritsch, Tobias Kießling,
Marc Großerüschkamp, Josef A. Käs: |
| Optical
Stretcher for Single Cells |
 Book
Description: This first modern book on the topic gives a broad overview
on current technology and application areas for single cell approaches
in life sciences.
Identification and imaging of single cells which is the most widely
applied single cell technology, is described in the opening section, including
fluorescence based methods, electron tomography and atomic force techniques.
The core section of the book covers a wide range of technologies for the
handling, manipulation and constituent analysis of individual cells. The
final section is dedicated to case studies and specific applications, with
examples ranging from cell biology and genetics to molecular medicine.
With its wide range to technologies and applications, this book is
the perfect companion for all scientists entering this growing field.
|
| Dario Anselmetti (Ed.): Single Cell Analysis - Technologies
and Applications, 1. Edition, Chapter 8 (161-174), Wiley-VCH, Weinheim
(2009) |
| Kristian Franze, Andreas Reichenbach, Josef Käs: |
| Biomechanics
of the CNS |
 Abstract
of Chapter 10: For a long time, neurosciences have focused on biochemical,
molecular, and electrophysiological aspects of cell functioning. However,
there is an increasing awareness of the importance of biomechanics in physiology
and pathology of the central nervous system (CNS). In the first part of
this review we provide physical basics necessary to understand biomechanical
measurements, we introduce the cytoskeleton as a major contributor to a
cell’s passive and active mechanical behavior, and we discuss some of the
methods nowadays used to quantify mechanical properties. In the second
part we present actual data on CNS mechanics, and we discuss the impact
of passive mechanical material properties and active mechanical behavior
of cells on the development, normal functioning and pathology of the CNS.
Book Description: This book presents the
latest findings in mechanosensitivity of the nervous system. The nervous
system stands out from a number of tissues because besides reacting to
the mechanical stress it is transmitting its own response to other organs
and tissues, which are located downstream of its signaling pathway. For
this reason any type of mechanical stimulation of the nervous system, which
is capable of triggering a physiological response, has high scientific
and practical significance, since it allows its use beyond a particular
experimental model anywhere where it is contributing to a particular pathological
condition.
This book is a unique collection of reviews outlining the current knowledge
and the future developments in this rapidly growing field. Currently, investigations
of the effects of mechanical stress on the nervous system are focused on
several issues. The majority of studies investigate the effects of mechanical
stimulation on mechanosensitive channels, as its primary target and interactive
agent, and aim on description of downstream intracellular signaling pathways
together with addressing general issues of biomechanics of the nervous
system. Knowledge of biomechanics, and mechanisms, which underlie it on
organism, organ, tissue and cellular level, is necessary for understanding
of the normal functioning of living organisms and allows to predict changes
which arise due to alterations of their environment, and possibly will
allow to develop new methods of artificial intervention.
The book brings up the problem closer to the experts in related medical
and biological sciences as well as practicing doctors besides just presenting
the latest achievements in the field.
|
| Andre Kamkin, Irina Kiseleva (Eds.): Mechanosensitivity
of the Nervous System, Series: Mechanosensitivity in Cells and Tissues
(Volume 2), 1. Edition, Chapter 10 (173-214), Springer Science+Business
Media B.V. (2008) |
| Bryan Lincoln, Falk Wottawah, Stefan Schinkinger, Susanne
Ebert, Jochen Guck: |
| High-Throughput
Rheological Measurements with an Optical Stretcher |
 Abstract
of Chapter 17: The cytoskeleton is a major determinant of the mechanical
strength and morphology of most cells. The composition and assembly state
of this intracellular polymer network evolve during the diVerentiation
of cells, and the structure is involved in many cellular functions and
is characteristically altered in many diseases, including cancer. Here
we exploit the deformability of the cytoskeleton as a link between molecular
structure and biological function, to distinguish between cells in diVerent
states by using a laser-based optical stretcher (OS) coupled with microfluidic
handling of cells. An OS is a cell-sized, dual-beam laser trap designed
to nondestructively test the deformability of single suspended cells. Combined
with microfluidic delivery, many cells can be measured serially in a short
amount of time.With this tool it could be shown that optical deformability
is sensitive enough to monitor subtle changes during the progression of
cells from normal to cancerous and even a metastatic state. Stem cells
can also be distinguished from more diVerentiated cells. The surprisingly
low number of cells required for this assay reflects the tight regulation
of the cytoskeleton by the cell. This suggests the possibility of using
optical deformability as an inherent cell marker for basic cell biological
investigation, diagnosis of disease, and sorting of stem cells from heterogeneous
populations, obviating the need for external markers or special preparation.
Many additional biological assays can be easily adapted to utilize this
innovative physical method. This chapter details the setup and use of the
microfluidic OS, the analysis and interpretation of data, and the results
of a typical experiment.
Book Description: Cell mechanics is the
field of study that looks at how cells detect, modify, and respond to the
physical properties of the cell environment. Cells communicate with each
other through chemical and physical signals which are involved in a range
of process from embryogenesis and wound healing to pathological conditions
such as cancerous invasion. Similar principles are also likely to be critical
for success in regenerative medicine. Cell mechanics is thus central to
understanding these principles. As cell mechanics draws from the fields
of biology, chemistry, physics, engineering, and mathematics, this book
aims not only to provide a collection of research methods, but also to
develop a common language among scientists who share the interest in cell
mechanics but enter the field with diverse backgrounds. To this end all
of the contributing authors have sought to explain in plain language the
nature of the biological problems, the rationale for the approaches, in
addition to the methods themselves. In addition, to balance practical utility
against conceptual advances, the book has intentionally included both chapters
that provide detailed recipes and those that emphasize basic principles.
|
| Yu-Li Wang, Dennis E. Discher (Eds.): Cell Mechanics,
Series: Methods in Cell Biology (Volume 83), 1. Edition, Chapter 17 (397-423),
Academic Press (2007) |
| Allen Ehrlicher, Timo Betz, Björn Stuhrmann, Michael
Gögler, Daniel Koch, Kristian Franze, Yun-Bi Lu, Josef Käs: |
| Optical
Neuronal Guidance |
| Abstract
of Chapter 21: We present a novel technique to noninvasively control
the growth and turning behavior of an extending neurite. A highly focused
infrared laser, positioned at the leading edge of a neurite, has been found
to induce extension/turning toward the beam's center. This technique has
been used successfully to guide NG108-15 and PC12 cell lines [Ehrlicher,
A., Betz, T., Stuhrmann, B., Koch, D. Milner, V. Raizen, M. G., and Kas,
J. (2002). Guiding neuronal growth with light. Proc. Natl. Acad. Sci. USA
99, 16024–16028], as well as primary rat and mouse cortical neurons [Stuhrmann,
B., Goegler, M., Betz, T., Ehrlicher, A., Koch, D., and Kas, J. (2005).
Automated tracking and laser micromanipulation of cells. Rev. Sci. Instr.
76, 035105]. Optical guidance may eventually be used alone or with other
methods for controlling neurite extension in both research and clinical
applications.
Book Description: Cell mechanics is the
field of study that looks at how cells detect, modify, and respond to the
physical properties of the cell environment. Cells communicate with each
other through chemical and physical signals which are involved in a range
of process from embryogenesis and wound healing to pathological conditions
such as cancerous invasion. Similar principles are also likely to be critical
for success in regenerative medicine. Cell mechanics is thus central to
understanding these principles. As cell mechanics draws from the fields
of biology, chemistry, physics, engineering, and mathematics, this book
aims not only to provide a collection of research methods, but also to
develop a common language among scientists who share the interest in cell
mechanics but enter the field with diverse backgrounds. To this end all
of the contributing authors have sought to explain in plain language the
nature of the biological problems, the rationale for the approaches, in
addition to the methods themselves. In addition, to balance practical utility
against conceptual advances, the book has intentionally included both chapters
that provide detailed recipes and those that emphasize basic principles.
|
| Yu-Li Wang, Dennis E. Discher (Eds.): Cell Mechanics,
Series: Methods in Cell Biology (Volume 83), 1. Edition, Chapter 21 (495-520),
Academic Press (2007) |
| Revathi Ananthakrishnan, Jochen Guck, Josef Kas: |
| Cell
Mechanics: Recent advances with a theoretical perspective |
| Abstract: N/A
|
| S.G. Pandalai (Ed.): Transworld Research Network: Recent
Research Developments in Biophysics, Volume 5, Part I, 39-69, Transworld
Research Network, Trivandrum, India (2006) |
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