for Biotechnology and Biomedicine
Institute of Bioanalytical Chemistry
Prof. Dr. Norbert Sträter
Hinge-bending domain movement of 5'-nucleotidase
The domain rotation observed for this enzyme differs markedly from other examples of domain movements in proteins. Here, the most common movement is a hinge-bending closure motion, by which the cleft between the two domains is opened and closed. In contrast, the rotation axis in 5'-NT (shown as a red arrow) passes close to the center of the C-terminal domain. As a result, this domain rotates approximately around its center. The residues at the domain interface slide along the interface, but the cleft between the two domains is not opened.