Endogenous GPCRs in Common Cell Lines

Agi Schonbrunn and David Steffen

CellReceptorPresentCommentReference
Most cells will probably contain:
 Adenosine A2BYes  
 Lysophosphatidic AcidYes  
 PGE (EP2 or EP4)Yes  
 ThrombinYes  
 UTPYes  
1321N1human astrocytoma
 muscarinic m3Yes Tang X, Wang L, Proud CG, Downes CP. Biochem J 374:137-43 (2003)
    Batty IH, Fleming IN, Downes CP. Biochem J 379:641-51 (2004)
 UTP P2Y2-likeYes Maurice Ho
AR4-2JRat pancreatic acinar cells
 bombesinYes Elberg G, Hipkin RW, Schonbrunn A. Mol Endocrinol 16:2502-14 (2002)
 cholecystokininYes Elberg G, Hipkin RW, Schonbrunn A. Mol Endocrinol 16:2502-14 (2002)
 somatostatin sst1No Gu YZ, Brown PJ, Loose-Mitchell DS, Stork PJ, Schonbrunn A. Mol Pharmacol 48:1004-14 (1996)
 somatostatin sst2Yes Elberg G, Hipkin RW, Schonbrunn A. Mol Endocrinol 16:2502-14 (2002)
    Gu YZ, Schonbrunn A. Mol Endocrinol 11:527-37 (1997)
AtT20mouse corticotrophic pituitary cells
 12-HETEYescoupled to cyclase inhibitionElliott M. Ross
 AngiotensinYes Elliott M. Ross
 Angiotensin AT1No Elliott M. Ross
 Corticotropin Releasing Hormone CRH-R1Yes Westendorf JM, Schonbrunn A. Endocrinology 116:2528-35 (1985)
 DynorphinYes Elliott M. Ross
 Histamine H3Yes Clark MA, Korte A, Egan RW. Agents Actions 40:129-34 (1994)
 Muscarinic m4Yes Elliott M. Ross
 OpioidNo Elliott M. Ross
 PACAP/VIP PVR2Yes Elliott M. Ross
 PACAP/VIP PVR3Yes Elliott M. Ross
 somatostatinYes Richardson UI, Schonbrunn A. Endocrinology 108:281-90 (1981)
 somatostatin sst1Yes Sarret P, Nouel D, Dal Farra C, Vincent JP, Beaudet A, Mazella J. J Biol Chem 274:19294-300 (1999)
 somatostatin sst2Yes Gu YZ, Schonbrunn A. Mol Endocrinol 11:527-37 (1997)
    Sarret P, Nouel D, Dal Farra C, Vincent JP, Beaudet A, Mazella J. J Biol Chem 274:19294-300 (1999)
 somatostatin sst5Yes Sarret P, Nouel D, Dal Farra C, Vincent JP, Beaudet A, Mazella J. J Biol Chem 274:19294-300 (1999)
 Substance P NK1Yes Elliott M. Ross
 VasopressinYes Elliott M. Ross
 VIPYes Westendorf JM, Schonbrunn A. Endocrinology 116:2528-35 (1985)
B10rat brain capillary endothelial cells
 Purinoceptor P2Y(T)Yes Webb TE, Feolde E, Vigne P, Neary JT, Runberg A, Frelin C, Barnard EA. Br J Pharmacol 119:1385-92 (1997)
    Simon J, Vigne P, Eklund KM, Michel AD, Carruthers AM, Humphrey PP, Frelin C, Barnard EA. Br J Pharmacol 132:173-82 (2001)
 Purinoceptor P2Y1Yes Vigne P, Breittmayer JP, Frelin C. Br J Pharmacol 129:1506-12 (2000)
B50mouse neuroblastoma
 Adrenergic Beta2Yes Ruck A, Millns P, Kendall DA, Hill SJ. Biochem Pharmacol 40:2371-5 (1990)
    McCrea KE, Hill SJ. Br J Pharmacol 110:619-26 (1993)
C6rat glial cells
 Adrenergic Beta1Yescoupled to PIP2 hydrolysisZhong H, Minneman KP. J Pharmacol Exp Ther 272:1088-94 (1995)
    McCrea KE, Hill SJ. Mol Pharmacol 49:927-37 (1996)
 Adrenergic Beta2Yes Zhong H, Minneman KP. J Pharmacol Exp Ther 272:1088-94 (1995)
    McCrea KE, Hill SJ. Mol Pharmacol 49:927-37 (1996)
    Ruck A, Millns P, Kendall DA, Hill SJ. Biochem Pharmacol 40:2371-5 (1990)
    Hosoda K, Fitzgerald LR, Vaidya VA, Feussner GK, Fishman PH, Duman RS. Mol Pharmacol 48:206-11 (1995)
 MuscarinicYescoupled to PIP2 hydrolysis 
 Purinoceptor P2Y(AC)=P2T(AC)Yes Jin J, Tomlinson W, Kirk IP, Kim YB, Humphries RG, Kunapuli SP. Br J Pharmacol 133:521-8 (2001)
 Serotonin 5HT2AYesBmax approx.100 fmol/mg proteinElliott JM, Newberry NR, Cholewinski AJ, Bartrup JT, Briddon SJ, Carey JE, Flanigan TP, Newton RA, Phipps SL, Reavley AC. Neuroscience 69:1119-31 (1996)
 Somatostatin sst2YesmRNAFeindt J, Becker I, Blomer U, Hugo HH, Mehdorn HM, Krisch B, Mentlein R. J Neurochem 65:1997-2005 (1995)
 Somatostatin sst3YesmRNAFeindt J, Becker I, Blomer U, Hugo HH, Mehdorn HM, Krisch B, Mentlein R. J Neurochem 65:1997-2005 (1995)
 Sphingosine 1-phosphateYes van Koppen C, Meyer zu Heringdorf M, Laser KT, Zhang C, Jakobs KH, Bunemann M, Pott L. J Biol Chem 271:2082-7 (1996)
    Sato K, Tomura H, Igarashi Y, Ui M, Okajima F. Mol Pharmacol 55:126-33 (1999)
CHO-K1Chinese Hamster Ovary Cells
 Adenosine A2BNo  
 Adrenergic Alpha2Yes<40 fmol/mg 
 Adrenergic BetaYesapprox. 30 fmol/mg protein 
 ATPYescoupled to calcium mobilization (at 10-5 M) 
 CalcitoninYescoupled to calcium mobilization & cyclase stimulationHorie K, Insel PA. J Biol Chem 275:29433-40 (2000)
 CCKMaybecoupled to PLC - controversial 
 Complement C3aYes Z-H Yan, Y Wang, TA Brock and AA Rege, Keystone Symposium on GPCRs 2004 Abstract 313
 corticotropin releasing factor CRF2Maybe100 nM CRF -> increase in cAMP 
 MuscarinicYes Wang SZ, Zhu SZ, el-Fakahany EE. Eur J Pharmacol 291:R1-2 (1996)
 Muscarinic m2Yes Wang SZ, Lee SY, Zhu SZ, Wotta DR, Parsons AM, el-Fakahany EE. Pharmacology 55:10-7 (1998)
 PAFYes  
 Serotonin 5-HT1BYes Giles H, Lansdell SJ, Bolofo ML, Wilson HL, Martin GR. Br J Pharmacol 117:1119-26 (1996)
 Sphingosine 1-phosphateYes van Koppen C, Meyer zu Heringdorf M, Laser KT, Zhang C, Jakobs KH, Bunemann M, Pott L. J Biol Chem 271:2082-7 (1996)
 Sphingosine-1-phosphate S1P2=Edg-5Yes Sandra Siehler
 ThrombinYescoupled to PLC 
 VIP Type IYeslow levels by binding 
COSAfrican green monkey kidney cells, SV40 transformed
 Adenosine A2BYes  
 Adrenergic BetaYes  
 Bradykinin B2Yesin COS-7 coupled to Ca mobilization Uwe Klein
 Dopamine D1-likeYesin COS-1: low density 
 Histamine H1Yescoupled to PLC in COS7Kuhn B, Schmid A, Harteneck C, Gudermann T, Schultz G. Mol Endocrinol 10:1697-707 (1997)
 Histamine H2No Kuhn B, Schmid A, Harteneck C, Gudermann T, Schultz G. Mol Endocrinol 10:1697-707 (1997)
 Lysophosphatidic AcidYes  
 melanocortinYes vijay.chhajlani@astrazeneca.com
 MuscarinicYes  
 PGE EP2Yes  
 Sphingosine 1-phosphateYesin COS-1van Koppen C, Meyer zu Heringdorf M, Laser KT, Zhang C, Jakobs KH, Bunemann M, Pott L. J Biol Chem 271:2082-7 (1996)
 ThrombinYes  
 UTP P2uYes  
GH3rat pituitary tumor cells
 Adenosine A1Yes Delahunty TM, Cronin MJ, Linden J. Biochem J 255:69-77 (1989)
 somatostatinYes Schonbrunn A, Tashjian H. J Biol Chem 253:6473-83 (1978)
 somatostatin sst1Yes Garcia PD, Myers RM. Mol Pharmacol 45:402-9 (1994)
 somatostatin sst2Yes Garcia PD, Myers RM. Mol Pharmacol 45:402-9 (1994)
 Thyrotropin releasing hormoneYes Hinkle PM, Tashjian AH. Biochemistry 14:3845-51 (1975)
GH4C1rat pituitary tumor cells
 Adenosine A1Yes Dorflinger LJ, Schonbrunn A. Endocrinology 117:2330-8 (1985)
    Zapata R, Navarro A, Canela EI, Franco R, Lluis C, Mallol J. J Neurochem 69:2546-54 (1997)
 bombesinYes Westendorf JM, Schonbrunn A. J Biol Chem 258:7527-35 (1983)
    Fischer JB, Schonbrunn A. J Biol Chem 263:2808-16 (1988)
 Growth hormone releasing hormoneNo  
 P2X7Yes Kimm-Brinson KL, Moeller PD, Barbier M, Glasgow H, Burkholder JM, Ramsdell JS. Environ Health Perspect 109:457-62 (2001)
 somatostatinYes Dorflinger LJ, Schonbrunn A. Endocrinology 113:1541-50 (1983)
    Koch BD, Schonbrunn A. Endocrinology 114:1784-90 (1984)
    Koch BD, Dorflinger LJ, Schonbrunn A. J Biol Chem 260:13138-45 (1985)
    Schonbrunn A, Tashjian H. J Biol Chem 253:6473-83 (1978)
    Dorflinger LJ, Schonbrunn A. Endocrinology 113:1551-8 (1983)
 somatostatin sst1Yes Gu YZ, Brown PJ, Loose-Mitchell DS, Stork PJ, Schonbrunn A. Mol Pharmacol 48:1004-14 (1996)
 somatostatin sst2Yes Gu YZ, Schonbrunn A. Mol Endocrinol 11:527-37 (1997)
 Thyrotropin Releasing HormoneYes Aizawa T, Hinkle PM. Endocrinology 116:73-82 (1985)
    Hinkle PM, Phillips WJ. Proc Natl Acad Sci U S A 81:6183-7 (1984)
 VIPYes Dorflinger LJ, Schonbrunn A. Endocrinology 113:1551-8 (1983)
HEK-293Human Embryonic Kidney Cells, adenovirus transformed
 Adenosine A2BYes Cooper J, Hill SJ, Alexander SP. Br J Pharmacol 122:546-50 (1997)
 Adrenergic Alpha 1No Theroux TL, Esbenshade TA, Peavy RD, Minneman KP. Mol Pharmacol 50:1376-87 (1996)
 Adrenergic Alpha2Maybe  
 Adrenergic BetaYesApprox. 7-20 fmol/mg protein. Coupled to cyclase stimulationPremont RT, Chen J, Ma HW, Ponnapalli M, Iyengar R. Proc Natl Acad Sci U S A 89:9809-13 (1992)
    Krupinski J, Lehman TC, Frankenfield CD, Zwaagstra JC, Watson PA. J Biol Chem 267:24858-62 (1992)
 BradykininYescoupled to calcium mobilizationAnderson L, Alexander CL, Faccenda E, Eidne KA. Biochem J 311 ( Pt 2):385-92 (1995)
 EndothelinYes  
 Lysophosphatidic Acid Edg2Yes Alderton F, Sambi B, Tate R, Pyne NJ, Pyne S. Br J Pharmacol 134:6-9 (2001)
 MuscarinicYescoupled to cyclase InhibitionKrupinski J, Lehman TC, Frankenfield CD, Zwaagstra JC, Watson PA. J Biol Chem 267:24858-62 (1992)
    Estacion M, Li S, Sinkins WG, Gosling M, Bahra P, Poll C, Westwick J, Schilling WP. J Biol Chem 279:22047-56 (2004)
 P2Y1 and P2Y2Yes Werry TD, Christie MI, Dainty IA, Wilkinson GF, Willars GB. Br J Pharmacol 135:1199-208 (2002)
    Wirkner K, Schweigel J, Gerevich Z, Franke H, Allgaier C, Barsoumian EL, Draheim H, Illes P. Br J Pharmacol 141:141-51 (2004)
    Schachter JB, Sromek SM, Nicholas RA, Harden TK. Neuropharmacology 36:1181-7 (1998)
 P2Y13Yes Wirkner K, Schweigel J, Gerevich Z, Franke H, Allgaier C, Barsoumian EL, Draheim H, Illes P. Br J Pharmacol 141:141-51 (2004)
 PACAP Type1Yes  
 PGE EP2Yes  
 SomatostatinYescoupled to cyclase inhibitionKaupmann K, Bruns C, Hoyer D, Seuwen K, Lubbert H. FEBS Lett 331:53-9 (1993)
 Sphingosine 1-phosphateYesSphingosine-1-phosphate (nM-range) & Sphingosylphosphorylcholine (uM range) stimulate IP3 formation. PTX sensitivevan Koppen C, Meyer zu Heringdorf M, Laser KT, Zhang C, Jakobs KH, Bunemann M, Pott L. J Biol Chem 271:2082-7 (1996)
    zu Heringdorf DM, Vincent ME, Lipinski M, Danneberg K, Stropp U, Wang DA, Tigyi G, Jakobs KH. Cell Signal 15:677-87 (2003)
 Sphingosine 1-phosphate, Edg-3Yes Siehler S, Wang Y, Fan X, Windh RT, Manning DR. J Biol Chem 276:48733-9 (2001)
 Sphingosine 1-phosphate, Edg-5Yes Siehler S, Wang Y, Fan X, Windh RT, Manning DR. J Biol Chem 276:48733-9 (2001)
 ThrombinYescoupled to PLC stimulationSchmidt M, Nehls C, Rumenapp U, Jakobs KH. Mol Pharmacol 50:1038-46 (1996)
 UTP P2uYes  
 VIP Type IMaybe  
HelaHuman cervical carcinoma cell line
 Adrenergic Beta2Maybecoupled to cyclase. Can be induced with butyrateTallman JF, Smith CC, Henneberry RC. Proc Natl Acad Sci U S A 74:873-7 (1977)
 EndothelinMaybe  
 Histamine H1Yescoupled to PLC 
 Lysophosphatidic AcidYescoupled to PLC 
 MuscarinicYescoupled to PLC 
 PurineYescoupled to PLC 
 Sphingosine 1-phosphateYesSignal via Gq 
HL-60human acute promyelocytic leukemia
 chemokine CCR1Yes Bassam Damaj
 chemokine CCR3Yesafter Butyric Acid and/or alkaline pH differentiationBassam Damaj
 chemokine CXCR1Yes Bassam Damaj
 chemokine CXCR2YesDibutyryl cAMP-treated HL-60 cellsBassam Damaj
 chemokine CXCR4YesVitamin D3-treated HL-60, DMSO-treated HL-60Bassam Damaj
 Leukotriene B4 (LTB4)Yes Dasari VR, Jin J, Kunapuli SP. Immunopharmacology 48:157-63 (2000)
HT29human colorectal adenocarcinoma
 Adrenergic Alpha2Yes Jones SB, Bylund DB. J Biol Chem 263:14236-44 (1988)
 OpioidYes  
 VIPYes  
IMR-32human neuroblastoma
 Muscarinic m1Yes Kukkonen J, Ojala P, Nasman J, Hamalainen H, Heikkila J, Akerman KE. J Pharmacol Exp Ther 263:1487-93 (1993)
 Muscarinic m2Yes Kukkonen J, Ojala P, Nasman J, Hamalainen H, Heikkila J, Akerman KE. J Pharmacol Exp Ther 263:1487-93 (1993)
 Muscarinic m3No Kukkonen J, Ojala P, Nasman J, Hamalainen H, Heikkila J, Akerman KE. J Pharmacol Exp Ther 263:1487-93 (1993)
Jurkathuman acute T cell leukemia
 chemokine CXCR4Yes Bassam Damaj
K562 cellshuman erythroleukemia cell line
 CCR1No Soda Y, Shimizu N, Jinno A, Liu HY, Kanbe K, Kitamura T, Hoshino H. Biochem Biophys Res Commun 258:313-21 (1999)
 CCR2bNo Soda Y, Shimizu N, Jinno A, Liu HY, Kanbe K, Kitamura T, Hoshino H. Biochem Biophys Res Commun 258:313-21 (1999)
 CCR3No Soda Y, Shimizu N, Jinno A, Liu HY, Kanbe K, Kitamura T, Hoshino H. Biochem Biophys Res Commun 258:313-21 (1999)
 CCR5No Soda Y, Shimizu N, Jinno A, Liu HY, Kanbe K, Kitamura T, Hoshino H. Biochem Biophys Res Commun 258:313-21 (1999)
 CXCR4No Soda Y, Shimizu N, Jinno A, Liu HY, Kanbe K, Kitamura T, Hoshino H. Biochem Biophys Res Commun 258:313-21 (1999)
 Duffy Antigen DARCYes Bassam Damaj
 GPR1No Soda Y, Shimizu N, Jinno A, Liu HY, Kanbe K, Kitamura T, Hoshino H. Biochem Biophys Res Commun 258:313-21 (1999)
 Leukotriene B4 (LTB4)Yes Dasari VR, Jin J, Kunapuli SP. Immunopharmacology 48:157-63 (2000)
 PGE EP1Yescoupled to calcium 
 Thrombin PAR1Yescoupled to calcium 
 Thromboxane A2YesTP (ligand: U46619) coupled to calcium 
 UTP/ATPYescoupled to calcium 
L cellsmouse fibroblasts
 PGEYescoupled to adenylyl cyclase stimulationElliott M. Ross
Ltk-thymidine kinase (TK) deficient L cells
 AdenosineMaybe  
 ATPMaybe  
 Lysophosphatidic AcidYescoupled to PLC 
 UTP P2uYescoupled to PLC and Ca 
MDA MB-231human breast cancer line, ER negative
 Lysophosphatidic AcidYescoupled to calcium and cyclase inhibition 
 Sphingosine 1-phosphateYescoupled to calcium 
 Thrombin PAR1Yescoupled to calcium and AC inhibition 
MDCK cellscanine kidney epithelial cells
 Adrenergic Beta 2Yes Rugg EL, Simmons NL. Q J Exp Physiol 69:339-53 (1984)
NAL-GThuman neuroblastoma
 Adrenergic Alpha2Yes Lambert DG. Neurosci Lett 183:4-7 (1995)
Neuro-2Amouse neuroblastoma
 Opioid DeltaYes Elliott M. Ross
 somatostatin sst2Yes Benedet JL, Boyes DA, Nichols TM, Millner A. Br J Obstet Gynaecol 84:517-21 (1977)
    Koenig JA, Edwardson JM, Humphrey PP. Br J Pharmacol 120:52-9 (1997)
 VIP/PACAPYes Lelievre V, Pineau N, Du J, Wen CH, Nguyen T, Janet T, Muller JM, Waschek JA. J Biol Chem 273:19685-90 (1998)
NG108-15murine neuroblastoma x rat glioma hybrid
 Adenosine A2AYes Mochly-Rosen D, Chang FH, Cheever L, Kim M, Diamond I, Gordon AS. Nature 333:848-50 (1988)
 Adrenergic Alpha2Yes Louie AK, Bass ES, Zhan J, Law PY, Loh HH. J Pharmacol Exp Ther 253:401-7 (1990)
    Strassheim D, Malbon CC. J Biol Chem 269:14307-13 (1994)
 Adrenergic Alpha2BYescoupled to calcium mobilizationHolmberg CI, Kukkonen JP, Bischoff A, Nasman J, Courtney MJ, Michel MC, Akerman KE. Eur J Pharmacol 363:65-74 (1999)
 Muscarinic m2Maybe Yasuda RP, Ciesla W, Flores LR, Wall SJ, Li M, Satkus SA, Weisstein JS, Spagnola BV, Wolfe BB. Mol Pharmacol 43:149-57 (1993)
 Muscarinic m4Yes Lazareno S, Buckley NJ, Roberts FF. Mol Pharmacol 38:805-15 (1991)
 Opioid DeltaYes Louie AK, Bass ES, Zhan J, Law PY, Loh HH. J Pharmacol Exp Ther 253:401-7 (1990)
    Kenakin T. Trends Pharmacol Sci 16:188-92 (1995)
    Strassheim D, Malbon CC. J Biol Chem 269:14307-13 (1994)
 P2UYes Kennedy C, IJzerman A. Trends Pharmacol Sci 15:311-2 (1995)
    Matsuoka I, Zhou Q, Ishimoto H, Nakanishi H. Mol Pharmacol 47:855-62 (1995)
 P2X7 (P2Z)Yes Kaiho H, Matsuoka I, Kimura J, Nakanishi H. J Neurochem 70:951-7 (1998)
 P2Y1Yes Halbfinger E, Major DT, Ritzmann M, Ubl J, Reiser G, Boyer JL, Harden KT, Fischer B. J Med Chem 42:5325-37 (2000)
 PGE-1Yes Mochly-Rosen D, Chang FH, Cheever L, Kim M, Diamond I, Gordon AS. Nature 333:848-50 (1988)
 ProstanoidYes Milligan G, Kim GD, Mullaney I, Adie EJ. Mol Cell Biochem 149-150:213-6 (1996)
 secretinYes Gossen D, Tastenoy M, Robberecht P, Christophe J. Eur J Biochem 193:149-54 (1990)
 SomatostatinYes Rhie DJ, Sung JH, Ha US, Kim HJ, Min do S, Hahn SJ, Kim MS, Jo YH, Yoon SH. Brain Res 975:120-8 (2003)
    Beaumont V, Hepworth MB, Luty JS, Kelly E, Henderson G. J Biol Chem 273:33174-83 (1999)
 somatostatin sst2BYes Vanetti M, Kouba M, Wang X, Vogt G, Hollt V. FEBS Lett 311:290-4 (1992)
NIH 3T3Mouse Fibroblast
 BombesinYesGq coupled 
 Lysophosphatidic AcidYesGq coupled 
 Sphingosine 1-phosphateYesGq coupledvan Koppen C, Meyer zu Heringdorf M, Laser KT, Zhang C, Jakobs KH, Bunemann M, Pott L. J Biol Chem 271:2082-7 (1996)
 ThrombinYesGq coupled 
PC-12rat phaeochromocytoma
 Adrenergic Alpha2Yes Gatti et al. BiochemJ 255,753-760, 1988
 Bradykinin B2Yes Pandiella A, Meldolesi J. J Biol Chem 264:3122-30 (1989)
 Neuropeptide Y Y1Yes Schwartz TW, Sheikh SP, O'Hare MM. FEBS Lett 225:209-14 (1988)
 Pancreatic Polypeptide Y4Yes Schwartz TW, Sheikh SP, O'Hare MM. FEBS Lett 225:209-14 (1988)
S49mouse lymphoma
 Andrenergic Beta2Yes Clark RB, Friedman J, Kunkel MW, January BG, Shenolikar S. J Biol Chem 268:3245-50 (1993)
    Post SR, Aguila-Buhain O, Insel PA. J Biol Chem 271:895-900 (1996)
 PGEYescoupled to cyclase stimulationKrumins AM, Lapeyre JN, Clark RB, Barber R. Biochem Pharmacol 54:43-59 (1997)
 Somatostatin sst2Yes Dent P, Wang Y, Gu YZ, Wood SL, Reardon DB, Mangues R, Pellicer A, Schonbrunn A, Sturgill TW. Cell Signal 9:539-49 (1998)
    Thomas JM, Hoffman BB. Cell Signal 4:417-28 (1992)
SH-SY5Yhuman neuroblastoma
 Adrenergic Alpha2Yes Lameh J, Eiger S, Sadee W. Eur J Pharmacol 227:19-24 (1992)
 Muscarinic m1YesCoupled to calciumKukkonen J, Ojala P, Nasman J, Hamalainen H, Heikkila J, Akerman KE. J Pharmacol Exp Ther 263:1487-93 (1993)
 Muscarinic m2Yes Kukkonen J, Ojala P, Nasman J, Hamalainen H, Heikkila J, Akerman KE. J Pharmacol Exp Ther 263:1487-93 (1993)
 Muscarinic m3YesCoupled to calciumKukkonen J, Ojala P, Nasman J, Hamalainen H, Heikkila J, Akerman KE. J Pharmacol Exp Ther 263:1487-93 (1993)
 Opioid muYes Lameh J, Eiger S, Sadee W. Eur J Pharmacol 227:19-24 (1992)
 somatostatin sst2AYes Hashemi SH, Li JY, Ahlman H, Dahlstrom A. Neurochem Res 28:449-60 (2003)
SK-N-BE(2)human neuroblastoma
 Adrenergic Alpha2Yes Namir N, Polastron J, Allouche S, Hasbi A, Jauzac P. J Neurochem 68:1764-72 (1997)
 Bradykinin B2Yes Pandiella A, Meldolesi J. J Biol Chem 264:3122-30 (1989)
 Dopamine D2Yes Namir N, Polastron J, Allouche S, Hasbi A, Jauzac P. J Neurochem 68:1764-72 (1997)
 MuscarinicYesm2/m4Namir N, Polastron J, Allouche S, Hasbi A, Jauzac P. J Neurochem 68:1764-72 (1997)
 Opioid DeltaYes Namir N, Polastron J, Allouche S, Hasbi A, Jauzac P. J Neurochem 68:1764-72 (1997)
 serotonin 5-HT2Yes Watson et al. Br. J. Pharmacol., 123, 234P (1998)
SK-N-MChuman neuroblastoma
 Adrenergic AlphaYeslow levelsTheroux TL, Esbenshade TA, Peavy RD, Minneman KP. Mol Pharmacol 50:1376-87 (1996)
 Adrenergic BetaYes Curran PK, Fishman PH. Cell Signal 8:355-64 (1997)
 calcitonin gene related peptide CGRPYes Roh J, Chang CL, Bhalla A, Klein C, Hsu SY. J Biol Chem 279:7264-74 (2004)
 NPY Y1Yes vijay.chhajlani@astrazeneca.com
 Nueropeptide Y1Yes Grandt D, Schimiczek M, Rascher W, Feth F, Shively J, Lee TD, Davis MT, Reeve JR, Michel MC. Regul Pept 67:33-7 (1997)
 orexinYes Wieland HA, Soll RM, Doods HN, Stenkamp D, Hurnaus R, Lammle B, Beck-Sickinger AG. Eur J Biochem 269:1128-35 (2002)
Swiss 3T3mouse fibroblast
 BombesinYes Williams BY, Wang Y, Schonbrunn A. Mol Pharmacol 50:716-27 (1996)
    Fischer JB, Schonbrunn A. J Biol Chem 263:2808-16 (1988)
THP-1human acute monocytic leukemia
 chemokine CCR1Yes Bassam Damaj
 chemokine CCR2Yes Bassam Damaj
 V28Yes Bassam Damaj
U937human pro-monocytic cells
 Andrenergic Beta2Yes Paul-Eugene N, Kolb JP, Calenda A, Gordon J, Kikutani H, Kishimoto T, Mencia-Huerta JM, Braquet P, Dugas B. Mol Immunol 30:157-64 (1993)
 BombesinYescoupled to calcium mobilizationMaurice Ho
 Chemokine CCR1, CCR3, CCR5Yes Zella D, Barabitskaja O, Burns JM, Romerio F, Dunn DE, Revello MG, Gerna G, Reitz MS, Gallo RC, Weichold FF. Blood 91:4444-50 (1998)
 Leukotriene LTB4Yescoupled to calcium mobilization. The density of these can be modulated by differentiation of the cells with DMSOAdv. Prostaglandin, Thromboxane, Leukotriene Res 19:180-6,1989
    Dasari VR, Jin J, Kunapuli SP. Immunopharmacology 48:157-63 (2000)
 Leukotriene LTD4Yes Pollock K, Creba J. Cell Signal 2:563-8 (1991)
    Adv. Prostaglandin, Thromboxane, Leukotriene Res 19:180-6,1989