Junior Research Group:
Protein Engineering

Head: Dr. Thomas Greiner-Stöffele
E-Mail: tgs@uni-leipzig.de

	Curriculum Vitae
	Research Interests
	Publications
	Group

Curriculum Vitae

Since 10/2001
Head of Junior Research Group 'Protein Engineering', BBZ (Biotechnological-Biomedical Center), Institute of Analytical Chemistry, University of Leipzig, Germany.

08/2000 to 08/2001
Post-Doctoral Fellow / Research Scientist, Max-Planck-Institute for Biophysical Chemistry at Göttingen, department of Prof. Dr. M. Eigen / DIREVO Biotech AG, Köln

04/1997 to 07/2001
Doctoral Candidate Researcher,
Institute of Biochemistry, University of Leipzig, Germany,
Department of Prof. Dr. U. Hahn

Ph.D. Thesis title: ´Selection and Characterization of RNase-stable RNA: Substrate Binding to RNase T1.'

1992-1997
Undergraduate Student Biochemistry,
University of Leipzig, Germany.

Research Interests

The goals of our research is the application and development of methods for rational protein design and in vitro evolution of proteins. Currently, we are focusing two projects: the increase of the thermostabilty of exonuclease III from Escherichia coli and the change of the specificity of ribonuclease T1 from Aspergillus oryzae. The topics include the development of specific assays for each enzyme mostly using fluorescence correlation spectroscopy, the generation of diversity with mutation and recombination methods, the selection or screening of interesting variants and their further characterization.

Publications

Korn, K.*, Greiner-Stöffele, T.*, Hahn, U. (2001) Ribonuclease assays utilizing toluidine blue indicator plates, methylene blue, or fluorescence correlation spectroscopy. Methods Enzymol., 341, 142-153.

Greiner-Stöffele, T., Förster, H.-H., Hofmann, H.-J., Hahn, U. (2001) RNase-stable RNA: conformational parameters of the nucleic acid backbone for binding to RNase T1. Biol. Chem., 382, 1007-1017.

Greiner-Stöffele, T., Förster, H.-H., Hahn, U. (2000) Ribonuclease T1 cleaves RNA after guanosines within single-stranded gaps of any length. Nucleosides Nucleotides Nucleic Acids, 19, 1101-1109.

Greiner-Stöffele, T., Grunow, M., Hahn, U. (1996) A general ribonuclease assay using methylene blue. Anal. Biochem. 240, 24-28.

Group

Sven Pfeifer
Antje Weihmann

Claudia Feller
Susann Härtig
Mandy Miertschke
Ramona Schmiedel