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| Poster, Friday, 19:00 |
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| Influence of Calcium Signaling
on Biomechanics of Single Suspended Cells in the Optical Stretcher
Markus Gyger, Roland Stange, Daniel
Rose, Tobias Kießling, Mareike Zink, Josef A. Käs
University of Leipzig, Faculty of
Physics and Earth Sciences, Institute of Experimental Physics I, Soft Matter
Physics Division, Linnéstraße 5, 04103 Leipzig, Germany |
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Under physiological conditions many cells must react to mechanical stimuli.
This raises interesting questions regarding the mechanisms by which cells
register and respond to applied forces. For adherent cells focal adhesions
seem to play an important role in mechano-transduction. Also calcium, one
of the most important second messengers, is involved in a number of known
mechano-activated cell responses.
In the Optical Stretcher, a tool to probe global mechanical behavior
of single cells in suspension, cells are trapped by two anti-parallel laser
beams. By increasing the laser power the momentum transferred to the cell
surface causes visible deformations. In the presented study cells transfected
with the heat sensitive calcium channel TRPV1, were used as a model system
to elucidate the influence of calcium signals on the mechanical properties
of cells independent of focal adhesions. To this end techniques to visualize,
quench, and artificially induce calcium signals were combined with optical
stretching. |
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