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| Poster, Friday, 19:00 |
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| Cell encapsulation in alginate
hollow spheres: A step towards true three dimensional cell culture
Bibhu Ranjan Sarangi1,*,
Kevin Alessandri1,*, Nicolas Bremond2, Luc Fetler1,
Jerome Bibette2, Christophe Lamaze3, Pierre Nassoy1
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1
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Physicochimie Curie, UMR-168, Institut
Curie, Paris |
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Laboratoire Colloides et Matériaux
Divisés, UMR 7612, ESPCI, Paris |
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Traffic, Signaling and Delivery,
UMR-144 Institut curie, Paris |
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*
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Equally contributed to the work |
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| Contact:
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Conventional two dimensional cell cultures like in a petri dish, provide
an excellent system for understanding several biological phenomena such
as cell adhesion, motility, division. However these two dimensional
systems do not provide adequate understanding about collective cell behavior
at tissue scale. To address that, several methods have been used to culture
cells in a three dimensional geometry. Cell aggregates in the form
of multicellular spheroids are one such example. Another method which
has been successfully attempted is embedding cells in hydrogels. However
such systems have their own limitations in terms of preparation methods
and their end usability.
In our lab we have adapted the technology of the Flavor Pearls TM,
which has been developed for culinary applications. By replacing food with
cells, we have implemented a microfluidic-assisted device that allows us
to encapsulate and grow cells in hollow alginate spheres. Using various
microscopy techniques such as contrast-phase, confocal and two-photon,
we monitor cell growth inside the alginate capsules. As compared to the
hydrogel embedding methods our encapsulation technique is simple and versatile.
We are able to encapsulate several types of cells. We believe that our
cellular capsules may provide a simple way to produce multicellular spheroids
and investigate the physical and biological properties of model tissues.
Acknowledgement: The Authors greatly acknowledge the Nikon Imaging Centre
at the Institut Curie-CNRS |
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