They are almost everywhere – in food, washing powder, dishwashing detergent, cosmetic products, in medicines as well as in drinking and waste water: hormonally active compounds such as synthetic oestrogen derivatives, which are the main ingredient in hormonal contraceptives, or the “bulk chemical” bisphenol A (BPA), which is used for example in beverage bottles or food cans. Its harmful effects on humans and the environment have long been proven.
However, simple detection of endocrine disruptors for effective monitoring and reliable risk assessment of products and waters is a challenge due to the structural diversity of these substances. Existing analytical methods are usually based on complex laboratory-diagnostic procedures or do not reach the required detection limits. The new method developed by scientists at the Universities of Dresden and Leipzig could now remedy this situation, which is why a patent application has been filed.
“Our method detects hormonally active compounds using immobilized sulfotransferases and microparticles and includes a kit for detecting the compounds in food, cosmetics, water samples and much more. To this end, we have implemented this enzyme of oestrogen metabolism into a biosensor that serves as a “capture probe” for oestrogen-like compounds. Depending on the concentration of oestrogen-like compounds in the detection solution, the binding of microparticles to a biochip is impaired and thus even low concentrations of hormonally active substances can be detected quickly and easily,” explains Professor Tilo Pompe from Leipzig University.
“In particular, I would like to point out the modularity of implementing an oestrogen-metabolizing enzyme, as the approach is not limited to this specific enzyme, but also allows the use of other hormone-metabolizing or hormone-binding proteins in a multiplex assay. This could open new approaches to cover the whole complexity of evaluating hormonally active substances without animal testing,” adds Dr Kai Ostermann from Technische Universität Dresden.
Original publication in Biosensors & Bioelectronics:
“Biomimetic estrogen sensor based on soft colloidal probes, Biosensors and Bioelectronics”, doi.org/10.1016/j.bios.2021.113506
Created by: Susann Huster